DNA Sequencer (Applied Biosystems 3100)

    Project: Research project

    Project Details


    DESCRIPTION: (provided by applicant): This application requests funds for an
    Applied Biosystems 3100 capillary DNA sequencer. The sequencer will be used for
    high volume DNA sequencing and DNA fragment analysis. The sequencer will be
    operated by the Molecular and Cellular Biology Core of the Oregon Regional
    Primate Research Center (ORPRC) an institute of Oregon Health Sciences
    University (OHSU) and serve the entire, newly-created, OHSU West Campus located
    12 miles from the main campus. Needs of major users, who are supported by NIH
    R0l and Center Grants, will include analysis of T-cell function that will
    generate 19,000 sequences annually, analyses of T cell haplotypes that will
    generate 12,000 sizing reaction annually, analysis of subtracted cDNA libraries
    that will generate 2,500 sequencing reactions annually, analysis of microarrays
    and differential display that will generate 2,000 reactions annually. The
    sequencer will also support the newly established OHSU microarray facility by
    sequencing 5,000 cDNA clones annually and the function of the MCB core itself
    by 3,000 sequencing reactions annually. Minor users will require an additional
    5,000 sequencing reactions annually. Because of this demand a high throughput
    machine is requested. The 3100 is a highly automated 16 capillary machine
    capable of running 7 days a week, 24 hours per day, from 9 to 16 runs per
    capillary per day. To process samples efficiently, the following accessories
    are requested: (1) An Applied Biosystems model 9700 PCR machine with twin 384
    sample blocks to prepare samples rapidly enough to keep up with the capacity of
    the 3100. (2) The Applied Biosystems Comparative Sequencing Software to allow
    rapid assembly and identification of obtained DNA sequences. (3) The Applied
    Biosystems Gene Mapper software package for DNA fragment analysis. This
    software combined with the DNA sequencer will allow users to rapidly obtain and
    process the thousands of reactions necessary for their research programs. An
    internal advisory Committee consisting of the Principal Investigator and three
    major users will monitor use of the sequencer and develop guidelines for shared
    use of the instrument. The Principal Investigator in consultation with the
    advisory committee will establish procedures and oversee general operation of
    the sequencer. The Principal Investigator has headed the Molecular and Cellular
    Biology Core at the ORPRC for the previous seven years and has 6 years
    experience with automated sequencing. A full time, Master?s level technician
    with 6 years experience with automated sequencers will tend to day to day
    operation of the sequencer. Currently the only DNA sequencer on the OHSU West
    Campus is an outdated ABI373 that this machine will replace. Institutional
    commitment to this application includes providing full service contracts, all
    needed renovations, back up power (generator and UPS), software upgrades and
    salary support for the technical staff.
    Effective start/end date5/1/024/30/03


    • National Institutes of Health: $149,350.00


    • Medicine(all)


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