Project Details
Description
Intracellular pathogens, such as Salmonella typhimurium, can survive within
a macrophage and have evolved a number of mechanisms to resist the
bacteriocidal mechanisms within these professional phagocytes. We have
developed a simple in vitro assay and used it to identify Salmonella
mutants that cannot survive within the macrophage. Roughly 1% of the Tn10
insertion mutants we have screened with this assay are not able to survive
in the macrophage and all of these are avirulent as expected for a mutation
in a known virulence mechanism. Secondary phenotypes have been assigned to
46 mutants identified in this screen. Many of the mutations are located in
pathways that would be anticipated to be essential for survival in the
macrophage (eg. sensitivity to oxidants) whereas others either have no
secondary phenotypes or were not expected (sensitivity to complement
components). Understanding of the defects in these mutants will contribute
to the understanding of other clinically important and more complex
intracellular pathogens.
a macrophage and have evolved a number of mechanisms to resist the
bacteriocidal mechanisms within these professional phagocytes. We have
developed a simple in vitro assay and used it to identify Salmonella
mutants that cannot survive within the macrophage. Roughly 1% of the Tn10
insertion mutants we have screened with this assay are not able to survive
in the macrophage and all of these are avirulent as expected for a mutation
in a known virulence mechanism. Secondary phenotypes have been assigned to
46 mutants identified in this screen. Many of the mutations are located in
pathways that would be anticipated to be essential for survival in the
macrophage (eg. sensitivity to oxidants) whereas others either have no
secondary phenotypes or were not expected (sensitivity to complement
components). Understanding of the defects in these mutants will contribute
to the understanding of other clinically important and more complex
intracellular pathogens.
| Status | Finished |
|---|---|
| Effective start/end date | 4/1/87 → 11/30/13 |
Funding
- National Institutes of Health: $268,655.00
- National Institutes of Health: $352,944.00
- National Institutes of Health: $344,135.00
- National Institutes of Health: $464,054.00
- National Institutes of Health: $334,110.00
- National Institutes of Health: $423,256.00
- National Institutes of Health: $334,654.00
- National Institutes of Health: $344,650.00
- National Institutes of Health: $289,404.00
- National Institutes of Health: $421,326.00
- National Institutes of Health: $423,704.00
- National Institutes of Health: $122,488.00
- National Institutes of Health: $353,415.00
- National Institutes of Health: $226,604.00
- National Institutes of Health: $327,729.00
- National Institutes of Health: $396,047.00
- National Institutes of Health: $188,750.00
- National Institutes of Health: $315,942.00
ASJC
- Medicine(all)
- Immunology and Microbiology(all)
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