Abstract
μ-Opioid receptors play a critical role in the regulation of the female reproductive cycle, and estrogen modulates the coupling of μ-opioid receptors to a potassium channel in the basal hypothalarnus (BH) of the female guinea pig. Therefore, we ascertained the distribution of μ-opioid receptors in the BH with autoradiography using the μ-opioid selective agonist [3H]DAMGO. In addition, we investigated the effects of estrogen on DAMGO- or the GABA(B) receptor agonist baclofen-stimulated [35S]GTPγS binding in the BH. Based on the high density of μ-opioid receptors, but the lack of effects of estrogen on [35S]GTPγS binding, we conclude that μ- opioid receptor interaction with its G-protein is not the target of estrogen's actions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 341-346 |
| Number of pages | 6 |
| Journal | Brain Research |
| Volume | 791 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - Apr 27 1998 |
Funding
We would like to thank Martha Bosch and Qixu Liu for expert technical assistance and Christopher Breivogel for technical advice. We would also like to thank Dr. Steven K. Childers for helpful discussions. This work was supported by NIH grants DA00158 and DA00192 (RSDA) to MJK.
| Funders | Funder number |
|---|---|
| Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health | DA00192, DA00158 |
Keywords
- Baclofen
- DAMGO
- Estrogen
- G-protein
- GTPγS
- μ-Opioid receptor
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology
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