[19] DNA Synthesis in Toluene-Treated Cells of Escherichia coli

Robs E. Moses

Research output: Contribution to journalArticlepeer-review


Toluene treatment kills ceils of Escherichia coli and makes them permeable to molecules of low molecular weight. Despite the fact that these cells are no longer viable, they retain a number of their physiological functions and the ability to synthesize DNA, if supplied with the necessary substrates. This chapter describes the assay of DNA synthesis in toluene-treated cells based upon the incorporation of radioactively labeled deoxynucleotide residues into acid-insoluble polydeoxynucleotides. DNA synthesis in toluene-treated cells occurs in a normal manner if the reaction mixture contains Triton X-100, a nonionic detergent. One of the convincing arguments that DNA synthesis in the toluene-treated cell system duplicates active replication in vivo has been the temperature effect on temperature-sensitive replication mutants in vitro.

Original languageEnglish (US)
Pages (from-to)219-224
Number of pages6
JournalMethods in Enzymology
Issue numberC
StatePublished - Jan 1 1974

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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