2-Chloro-1,4-dimethoxybenzene cation radical: Formation and role in the lignin peroxidase oxidation of anisyl alcohol

Pauline J.M. Teunissen, Dawei Sheng, G. Vijay Bhasker Reddy, Pierre Moënne-Loccoz, Jim A. Field, Michael H. Gold

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


2-Chloro-1,4-dimethoxybenzene (2Cl-1,4-DMB) oxidation by lignin peroxidase (LIP) proceeds via the formation of the 2Cl-1,4-DMB cation radical as indicated by ESR and UV/vis spectroscopy. The products of the LiP- catalyzed oxidation of 2Cl-1,4-DMB were identified as 2-chloro-1,4- benzoquinone and the dimers dichlorotetramethoxybiphenyl and chloro(chlorodimethoxyphenyl)benzoquinone. The addition of anisyl alcohol (AA) rapidly quenched the 2Cl-1,4-DMB cation radical optical absorption bands, suggesting that the cation radical directly mediates the oxidation of AA. When LiP reactions are conducted in the presence of 50 μM 2Cl-1,4-DMB, the enzyme is inactivated; however, this inactivation can be prevented by the addition of AA. This also suggests that the 2Cl-1,4-DMB cation radical formed in the reaction, in turn, oxidizes AA.

Original languageEnglish (US)
Pages (from-to)233-238
Number of pages6
JournalArchives of Biochemistry and Biophysics
Issue number2
StatePublished - Dec 15 1998


  • 2-chloro-1,4-dimethoxybenzene
  • Anisyl alcohol
  • Cation radical
  • Lignin peroxidase
  • Mediator

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology


Dive into the research topics of '2-Chloro-1,4-dimethoxybenzene cation radical: Formation and role in the lignin peroxidase oxidation of anisyl alcohol'. Together they form a unique fingerprint.

Cite this