A Broccoli aptamer chimera yields a fluorescent K+sensor spanning physiological concentrations

Jonathan C. Savage; Pushkar Shinde; Yizhou Yao; Monika A. Davare; Ujwal Shinde

doi:10.1039/d0cc07042d

A Broccoli aptamer chimera yields a fluorescent K⁺sensor spanning physiological concentrations

Jonathan C. Savage, Pushkar Shinde, Yizhou Yao, Monika A. Davare, Ujwal Shinde

Pediatric Hematology and Oncology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

The RNA aptamer Broccoli accepts 2′fluorinated (2′F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K⁺>30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K⁺concentrations, yielding an effective RNA-based fluorescent K⁺sensor.

Original language	English (US)
Pages (from-to)	1344-1347
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	11
DOIs	https://doi.org/10.1039/d0cc07042d
State	Published - Feb 7 2021

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

Access to Document

10.1039/d0cc07042d

Cite this

@article{fe38f5e04c7f439ea71c96208f550837,

title = "A Broccoli aptamer chimera yields a fluorescent K+sensor spanning physiological concentrations",

abstract = "The RNA aptamer Broccoli accepts 2′fluorinated (2′F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K+>30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K+concentrations, yielding an effective RNA-based fluorescent K+sensor.",

author = "Savage, {Jonathan C.} and Pushkar Shinde and Yizhou Yao and Davare, {Monika A.} and Ujwal Shinde",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",

year = "2021",

month = feb,

day = "7",

doi = "10.1039/d0cc07042d",

language = "English (US)",

volume = "57",

pages = "1344--1347",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "11",

}

TY - JOUR

T1 - A Broccoli aptamer chimera yields a fluorescent K+sensor spanning physiological concentrations

AU - Savage, Jonathan C.

AU - Shinde, Pushkar

AU - Yao, Yizhou

AU - Davare, Monika A.

AU - Shinde, Ujwal

N1 - Publisher Copyright: © The Royal Society of Chemistry 2021.

PY - 2021/2/7

Y1 - 2021/2/7

N2 - The RNA aptamer Broccoli accepts 2′fluorinated (2′F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K+>30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K+concentrations, yielding an effective RNA-based fluorescent K+sensor.

AB - The RNA aptamer Broccoli accepts 2′fluorinated (2′F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K+>30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K+concentrations, yielding an effective RNA-based fluorescent K+sensor.

UR - http://www.scopus.com/inward/record.url?scp=85101156347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85101156347&partnerID=8YFLogxK

U2 - 10.1039/d0cc07042d

DO - 10.1039/d0cc07042d

M3 - Article

C2 - 33432937

AN - SCOPUS:85101156347

SN - 1359-7345

VL - 57

SP - 1344

EP - 1347

JO - Chemical Communications

JF - Chemical Communications

IS - 11

ER -

A Broccoli aptamer chimera yields a fluorescent K⁺sensor spanning physiological concentrations

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this