A FlAsH-based cross-linker to study protein interactions in living cells

Anna Rutkowska; Christian H. Haering; Carsten Schultz

doi:10.1002/anie.201106404

A FlAsH-based cross-linker to study protein interactions in living cells

Anna Rutkowska, Christian H. Haering, Carsten Schultz

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

As you like it: xCrAsH, a dimeric derivative of the arsenical compound FlAsH, enables the highly specific, covalent cross-linking of two proteins containing a 12 amino acid peptide tag. This inducible and (by addition of dithiols) reversible system can be used to detect and manipulate protein-protein interactions both in vitro and in living cells (see picture).

Original language	English (US)
Pages (from-to)	12655-12658
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	52
DOIs	https://doi.org/10.1002/anie.201106404
State	Published - Dec 23 2011
Externally published	Yes

Keywords

biarsenical compounds
dimerization
protein cross-linking
protein-protein interactions
small molecules

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201106404

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abstract = "As you like it: xCrAsH, a dimeric derivative of the arsenical compound FlAsH, enables the highly specific, covalent cross-linking of two proteins containing a 12 amino acid peptide tag. This inducible and (by addition of dithiols) reversible system can be used to detect and manipulate protein-protein interactions both in vitro and in living cells (see picture).",

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AU - Schultz, Carsten

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AB - As you like it: xCrAsH, a dimeric derivative of the arsenical compound FlAsH, enables the highly specific, covalent cross-linking of two proteins containing a 12 amino acid peptide tag. This inducible and (by addition of dithiols) reversible system can be used to detect and manipulate protein-protein interactions both in vitro and in living cells (see picture).

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KW - small molecules

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A FlAsH-based cross-linker to study protein interactions in living cells

Abstract

Keywords

ASJC Scopus subject areas

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