A high-resolution PAC and BAC map of the SCA2 region

Tamilla Nechiporuk; Alex Nechiporuk; Soodabeh Sahba; Karla Figueroa; Hiroki Shibata; Xiao Ning Chen; Julie R. Korenberg; Pieter De Jong; Stefan M. Pulst

doi:10.1006/geno.1997.4886

A high-resolution PAC and BAC map of the SCA2 region

Tamilla Nechiporuk
, Alex Nechiporuk
, Soodabeh Sahba
, Karla Figueroa
, Hiroki Shibata
, Xiao Ning Chen
, Julie R. Korenberg
, Pieter De Jong
, Stefan M. Pulst

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

The spinocerebellar ataxia type 2 (SCA2) gene has been localized to chromosome 12q24.1. To characterize this region and to aid in the identification of the SCA2 gene, we have constructed a 3.9-Mb physical map, which covers markers D12S1328 and D12S1329 known to flank the gene. The map comprises a contig of 84 overlapping yeast artificial chromosomes (YACs), P1 artificial chromosomes (PACs), and bacterial artificial chromosomes (BACs) onto which we placed 82 PCR markers. We localized eight genes and expressed sequence tags on this map, many of which had not been precisely mapped before. In contrast to YACs, which showed a high degree of chimerism and deletions in this region, PACs and BACs were stable. Only 1 in 65 PACs contained a small deletion, and 2 in 18 BACs were chimeric. The high- resolution physical map, which was used in the identification of the SCA2 gene, will be useful for the positional cloning of other disease genes mapped to this region.

Original language	English (US)
Pages (from-to)	321-329
Number of pages	9
Journal	Genomics
Volume	44
Issue number	3
DOIs	https://doi.org/10.1006/geno.1997.4886
State	Published - Sep 15 1997
Externally published	Yes

Funding

We thank T. Hudson for the gift of YACs Y884_h_11, Y919_a_2, Y792_b_4, Y731_b_7, and Y856_h_2. We thank D. Scoles for the careful review of the manuscript. This work was supported by the Carmen and Louis Warschaw Endowment Fund for Neurology, the Ruth and Lawrence Harvey Endowment Fund for the Neurosciences, and the National Institutes of Health (all to S.-M.P.).

Funders
Carmen and Louis Warschaw Endowment Fund for Neurology
Ruth and Lawrence Harvey Endowment Fund for the Neurosciences
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health

ASJC Scopus subject areas

Genetics

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10.1006/geno.1997.4886

Cite this

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title = "A high-resolution PAC and BAC map of the SCA2 region",

abstract = "The spinocerebellar ataxia type 2 (SCA2) gene has been localized to chromosome 12q24.1. To characterize this region and to aid in the identification of the SCA2 gene, we have constructed a 3.9-Mb physical map, which covers markers D12S1328 and D12S1329 known to flank the gene. The map comprises a contig of 84 overlapping yeast artificial chromosomes (YACs), P1 artificial chromosomes (PACs), and bacterial artificial chromosomes (BACs) onto which we placed 82 PCR markers. We localized eight genes and expressed sequence tags on this map, many of which had not been precisely mapped before. In contrast to YACs, which showed a high degree of chimerism and deletions in this region, PACs and BACs were stable. Only 1 in 65 PACs contained a small deletion, and 2 in 18 BACs were chimeric. The high- resolution physical map, which was used in the identification of the SCA2 gene, will be useful for the positional cloning of other disease genes mapped to this region.",

author = "Tamilla Nechiporuk and Alex Nechiporuk and Soodabeh Sahba and Karla Figueroa and Hiroki Shibata and Chen, \{Xiao Ning\} and Korenberg, \{Julie R.\} and \{De Jong\}, Pieter and Pulst, \{Stefan M.\}",

note = "Funding Information: We thank T. Hudson for the gift of YACs Y884\_h\_11, Y919\_a\_2, Y792\_b\_4, Y731\_b\_7, and Y856\_h\_2. We thank D. Scoles for the careful review of the manuscript. This work was supported by the Carmen and Louis Warschaw Endowment Fund for Neurology, the Ruth and Lawrence Harvey Endowment Fund for the Neurosciences, and the National Institutes of Health (all to S.-M.P.).",

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AU - Shibata, Hiroki

AU - Chen, Xiao Ning

AU - Korenberg, Julie R.

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AU - Pulst, Stefan M.

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N2 - The spinocerebellar ataxia type 2 (SCA2) gene has been localized to chromosome 12q24.1. To characterize this region and to aid in the identification of the SCA2 gene, we have constructed a 3.9-Mb physical map, which covers markers D12S1328 and D12S1329 known to flank the gene. The map comprises a contig of 84 overlapping yeast artificial chromosomes (YACs), P1 artificial chromosomes (PACs), and bacterial artificial chromosomes (BACs) onto which we placed 82 PCR markers. We localized eight genes and expressed sequence tags on this map, many of which had not been precisely mapped before. In contrast to YACs, which showed a high degree of chimerism and deletions in this region, PACs and BACs were stable. Only 1 in 65 PACs contained a small deletion, and 2 in 18 BACs were chimeric. The high- resolution physical map, which was used in the identification of the SCA2 gene, will be useful for the positional cloning of other disease genes mapped to this region.

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A high-resolution PAC and BAC map of the SCA2 region

Abstract

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