@inproceedings{732ba2c4057b483f993482da7bfe6c15,
title = "A method for validating depth-resolved biodistributions in topically-stained specimen with multi-channel fluorescence cryo-imaging.",
abstract = "Fluorescent contrast agents targeted to cancer biomarkers are increasingly being explored for cancer detection, surgical guidance, and response monitoring. Efforts have been underway to topically apply such biomarker-targeted agents to freshly excised specimen for detecting cancer cell receptors on the surface as a method for intraoperative surgical margin assessment, including dual-probe staining methods introduce a second {\textquoteleft}non-specific{\textquoteright} optical agent as a control to help compensate for heterogeneous uptake and normalize the imaging field. Still, such specimen staining protocols introduce multifaceted complexity with unknown variables, such as tissue-specific diffusion, cell-specific binding and disassociation rates, and other factors, affecting the interpreted cancer-biomarker distribution across the specimen surface. The ability to recover three-dimensional dual-probe biodistributions throughout whole-specimens could offer a ground-truth validation method for examining topical staining uptake behaviors. Herein, we report on a novel method for characterizing dual-probe accumulation with 3D depth-profiles observed from a dual-probe fresh-specimen staining experiment.",
keywords = "Cryo-macrotome, Drug biodistribution, Molecular imaging",
author = "Byrd, {Brook K.} and Strawbridge, {Rendall S.} and Wendy Wells and Connor Barth and Summer Gibbs and Davis, {Scott C.}",
note = "Publisher Copyright: {\textcopyright} 2021 SPIE.; Molecular-Guided Surgery: Molecules, Devices, and Applications VII 2021 ; Conference date: 06-03-2021 Through 11-03-2021",
year = "2021",
doi = "10.1117/12.2582542",
language = "English (US)",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
editor = "Sylvain Gioux and Gibbs, {Summer L.} and Pogue, {Brian W.}",
booktitle = "Molecular-Guided Surgery",
}