A monoclonal antibody that binds to photoreceptors in the turtle retina

Vinod P. Gaur; Grazyna Adamus; Anatol Arendt; William Eldred; Daniel E. Possin; J. Hugh McDowell; Paul A. Hargrave; P. Vijay Sarthy

doi:10.1016/0042-6989(88)90023-5

A monoclonal antibody that binds to photoreceptors in the turtle retina

Vinod P. Gaur, Grazyna Adamus, Anatol Arendt, William Eldred, Daniel E. Possin, J. Hugh McDowell, Paul A. Hargrave, P. Vijay Sarthy

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We have raised monoclonal antibodies to photoreceptor cells in the retina of the turtle (Pseudemys scripta elegans). One of these antibodies, 15-18 (an IgG₁), was studied by immunoelectron microscopy using colloidal gold, and found to bind to the outer segments of all rods and some single cones, but did not stain turtle double cones. Immunoblotting and immunoprecipitation show that antibody 15-18 binds to an antigen of apparent M_r ~ 34,5000 which is probably turtle opsin. Antibody 15-18 binds visual pigments from several species, including bovine opsin. In order to determine the antigenic site bound by 15-18 in bovine opsin, synthetic peptides were used as competitors in an enzyme-linked immunoassay (ELISA). The antigenic site is located in the surface loop connecting rhodopsin helices IV-V, in the sequence 190-197. Antibody 15-18 binds to the external surface of rod cell outer segments, thus providing direct evidence for the predicted orientation of rhodopsin in the plasma membrane.

Original language	English (US)
Pages (from-to)	765-776
Number of pages	12
Journal	Vision Research
Volume	28
Issue number	7
DOIs	https://doi.org/10.1016/0042-6989(88)90023-5
State	Published - 1988
Externally published	Yes

Keywords

Cone
Monoclonal antibodies
Rod
Turtle

ASJC Scopus subject areas

Ophthalmology
Sensory Systems

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10.1016/0042-6989(88)90023-5

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@article{8a498248d8cb406c8d2b6ae8a60f3a04,

title = "A monoclonal antibody that binds to photoreceptors in the turtle retina",

abstract = "We have raised monoclonal antibodies to photoreceptor cells in the retina of the turtle (Pseudemys scripta elegans). One of these antibodies, 15-18 (an IgG1), was studied by immunoelectron microscopy using colloidal gold, and found to bind to the outer segments of all rods and some single cones, but did not stain turtle double cones. Immunoblotting and immunoprecipitation show that antibody 15-18 binds to an antigen of apparent Mr ~ 34,5000 which is probably turtle opsin. Antibody 15-18 binds visual pigments from several species, including bovine opsin. In order to determine the antigenic site bound by 15-18 in bovine opsin, synthetic peptides were used as competitors in an enzyme-linked immunoassay (ELISA). The antigenic site is located in the surface loop connecting rhodopsin helices IV-V, in the sequence 190-197. Antibody 15-18 binds to the external surface of rod cell outer segments, thus providing direct evidence for the predicted orientation of rhodopsin in the plasma membrane.",

keywords = "Cone, Monoclonal antibodies, Rod, Turtle",

author = "Gaur, {Vinod P.} and Grazyna Adamus and Anatol Arendt and William Eldred and Possin, {Daniel E.} and McDowell, {J. Hugh} and Hargrave, {Paul A.} and Sarthy, {P. Vijay}",

note = "Funding Information: Acknowledgemenfs-We thank Dr Ann Bunt-Milam for enthusiastich elp throughoutt he courseo f this work. The able, technicala ssistanceo f Felicitas Eldred is gratefully acknowledgedW. e also thank Ron Jones and Brad Clifton for photographich elp.W ork at the Universityo f Washington was supported in part by NIH Research Grants EY03664,E Y03523,E Y01730,E Y07013,E Y04785,a nd by an award from Researcht o PreventB lindnessI nc. Some facilitiesw eres upportedin part by HD02274.W ork at the University of Florida was supportedi n part by NIH Re-searchG rants EY06225a nd EY06226,a Jules and Doris SteinP rofessorshipfr om Researchto PreventB lindnessIn c. (to P.A.H.), and an unrestrictedd epartmentaalw ardfrom Researcht o PreventB lindnessI nc.",

year = "1988",

doi = "10.1016/0042-6989(88)90023-5",

language = "English (US)",

volume = "28",

pages = "765--776",

journal = "Vision Research",

issn = "0042-6989",

publisher = "Elsevier Limited",

number = "7",

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TY - JOUR

T1 - A monoclonal antibody that binds to photoreceptors in the turtle retina

AU - Gaur, Vinod P.

AU - Adamus, Grazyna

AU - Arendt, Anatol

AU - Eldred, William

AU - Possin, Daniel E.

AU - McDowell, J. Hugh

AU - Hargrave, Paul A.

AU - Sarthy, P. Vijay

N1 - Funding Information: Acknowledgemenfs-We thank Dr Ann Bunt-Milam for enthusiastich elp throughoutt he courseo f this work. The able, technicala ssistanceo f Felicitas Eldred is gratefully acknowledgedW. e also thank Ron Jones and Brad Clifton for photographich elp.W ork at the Universityo f Washington was supported in part by NIH Research Grants EY03664,E Y03523,E Y01730,E Y07013,E Y04785,a nd by an award from Researcht o PreventB lindnessI nc. Some facilitiesw eres upportedin part by HD02274.W ork at the University of Florida was supportedi n part by NIH Re-searchG rants EY06225a nd EY06226,a Jules and Doris SteinP rofessorshipfr om Researchto PreventB lindnessIn c. (to P.A.H.), and an unrestrictedd epartmentaalw ardfrom Researcht o PreventB lindnessI nc.

PY - 1988

Y1 - 1988

N2 - We have raised monoclonal antibodies to photoreceptor cells in the retina of the turtle (Pseudemys scripta elegans). One of these antibodies, 15-18 (an IgG1), was studied by immunoelectron microscopy using colloidal gold, and found to bind to the outer segments of all rods and some single cones, but did not stain turtle double cones. Immunoblotting and immunoprecipitation show that antibody 15-18 binds to an antigen of apparent Mr ~ 34,5000 which is probably turtle opsin. Antibody 15-18 binds visual pigments from several species, including bovine opsin. In order to determine the antigenic site bound by 15-18 in bovine opsin, synthetic peptides were used as competitors in an enzyme-linked immunoassay (ELISA). The antigenic site is located in the surface loop connecting rhodopsin helices IV-V, in the sequence 190-197. Antibody 15-18 binds to the external surface of rod cell outer segments, thus providing direct evidence for the predicted orientation of rhodopsin in the plasma membrane.

AB - We have raised monoclonal antibodies to photoreceptor cells in the retina of the turtle (Pseudemys scripta elegans). One of these antibodies, 15-18 (an IgG1), was studied by immunoelectron microscopy using colloidal gold, and found to bind to the outer segments of all rods and some single cones, but did not stain turtle double cones. Immunoblotting and immunoprecipitation show that antibody 15-18 binds to an antigen of apparent Mr ~ 34,5000 which is probably turtle opsin. Antibody 15-18 binds visual pigments from several species, including bovine opsin. In order to determine the antigenic site bound by 15-18 in bovine opsin, synthetic peptides were used as competitors in an enzyme-linked immunoassay (ELISA). The antigenic site is located in the surface loop connecting rhodopsin helices IV-V, in the sequence 190-197. Antibody 15-18 binds to the external surface of rod cell outer segments, thus providing direct evidence for the predicted orientation of rhodopsin in the plasma membrane.

KW - Cone

KW - Monoclonal antibodies

KW - Rod

KW - Turtle

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UR - http://www.scopus.com/inward/citedby.url?scp=0023881021&partnerID=8YFLogxK

U2 - 10.1016/0042-6989(88)90023-5

DO - 10.1016/0042-6989(88)90023-5

M3 - Article

C2 - 3067443

AN - SCOPUS:0023881021

SN - 0042-6989

VL - 28

SP - 765

EP - 776

JO - Vision Research

JF - Vision Research

IS - 7

ER -

A monoclonal antibody that binds to photoreceptors in the turtle retina

Abstract

Keywords

ASJC Scopus subject areas

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