TY - JOUR
T1 - A monokine regulates colony-stimulating activity production by vascular endothelial cells
AU - Bagby, G. C.
AU - McCall, E.
AU - Bergstrom, K. A.
AU - Burger, D.
PY - 1983
Y1 - 1983
N2 - Human umbilical vein endothelial cells were cultured in supernatants of peripheral blood monocytes that had been cultured for 3 days with and without lactoferrin. Colony-stimulating activity (CSA) was measured in supernatants of the endothelial cell cultures and appropriate control cultures using normal T-lymphocyte-depleted, phagocyte-depleted, low-density bone marrow cells in colony growth (CFU-GM) assays. Monocyte-conditioned medium contained a nondialyzable, heat labile factor that enhanced 4-15 fold the production of CSA by endothelial cells. The addition of lactoferrin to monocyte cultures reduced the activity of this monokine by 69%. Lactoferrin did not inhibit CSA production by monokine-stimulated endothelial cells. Therefore, the production of CSA by these cells is regulated by a stimulatory monokine, and the production and/or release of the monokinase is inhibited by lactoferrin, a neutrophil-derived putative feedback inhibitor of granulopoiesis. Inasmuch as a similar monokine is known to stimulate CSA production by fibroblasts and T lymphocytes, we suggest that mononuclear phagocytes play a pivotal role in the regulation of granulopoiesis by recruiting a variety of cell types to produce CSA.
AB - Human umbilical vein endothelial cells were cultured in supernatants of peripheral blood monocytes that had been cultured for 3 days with and without lactoferrin. Colony-stimulating activity (CSA) was measured in supernatants of the endothelial cell cultures and appropriate control cultures using normal T-lymphocyte-depleted, phagocyte-depleted, low-density bone marrow cells in colony growth (CFU-GM) assays. Monocyte-conditioned medium contained a nondialyzable, heat labile factor that enhanced 4-15 fold the production of CSA by endothelial cells. The addition of lactoferrin to monocyte cultures reduced the activity of this monokine by 69%. Lactoferrin did not inhibit CSA production by monokine-stimulated endothelial cells. Therefore, the production of CSA by these cells is regulated by a stimulatory monokine, and the production and/or release of the monokinase is inhibited by lactoferrin, a neutrophil-derived putative feedback inhibitor of granulopoiesis. Inasmuch as a similar monokine is known to stimulate CSA production by fibroblasts and T lymphocytes, we suggest that mononuclear phagocytes play a pivotal role in the regulation of granulopoiesis by recruiting a variety of cell types to produce CSA.
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U2 - 10.1182/blood.v62.3.663.663
DO - 10.1182/blood.v62.3.663.663
M3 - Article
C2 - 6603879
AN - SCOPUS:0020637487
SN - 0006-4971
VL - 62
SP - 663
EP - 668
JO - Blood
JF - Blood
IS - 3
ER -