A new DNA polymerase activity of Escherichia coli. II. Properties of the enzyme purified from wild-type E. coli and DNAts mutants

Robb E. Moses; Charles C. Richardson

doi:10.1016/0006-291X(70)90566-8

A new DNA polymerase activity of Escherichia coli. II. Properties of the enzyme purified from wild-type E. coli and DNA_ts mutants

Robb E. Moses
, Charles C. Richardson

Molecular and Medical Genetics

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

DNA polymerases I and II have been purified from wild-type Escherichia coli and from two mutants of E. coli. Polymerase II is present in wild-type cells in an amount identical to that present in E. coli polAl. In two DNA_ts mutants, CR43-26 and CR266-26, both of the "fast stop" variety, neither polymerase activity is temperature sensitive in vitro.

Original language	English (US)
Pages (from-to)	1565-1571
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	41
Issue number	6
DOIs	https://doi.org/10.1016/0006-291X(70)90566-8
State	Published - Dec 24 1970

Funding

Acknowledgement. The work described by grants from National Institutes from the American Cancer Society (P-486). R.E.M. was supported by a Public Health Service Fellowship (5-F03-GM42,968). C.C.R. is the recipient of a Public Health Service Research Career Program Award (GM-13,634).

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health
U.S. Public Health Service	5-F03-GM42,968, GM-13,634

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(70)90566-8

Cite this

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title = "A new DNA polymerase activity of Escherichia coli. II. Properties of the enzyme purified from wild-type E. coli and DNAts mutants",

abstract = "DNA polymerases I and II have been purified from wild-type Escherichia coli and from two mutants of E. coli. Polymerase II is present in wild-type cells in an amount identical to that present in E. coli polAl. In two DNAts mutants, CR43-26 and CR266-26, both of the {"}fast stop{"} variety, neither polymerase activity is temperature sensitive in vitro.",

author = "Moses, \{Robb E.\} and Richardson, \{Charles C.\}",

note = "Funding Information: Acknowledgement. The work described by grants from National Institutes Funding Information: from the American Cancer Society (P-486). R.E.M. was supported by a Public Health Service Fellowship (5-F03-GM42,968). C.C.R. is the recipient of a Public Health Service Research Career Program Award (GM-13,634).",

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AU - Moses, Robb E.

AU - Richardson, Charles C.

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Y1 - 1970/12/24

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AB - DNA polymerases I and II have been purified from wild-type Escherichia coli and from two mutants of E. coli. Polymerase II is present in wild-type cells in an amount identical to that present in E. coli polAl. In two DNAts mutants, CR43-26 and CR266-26, both of the "fast stop" variety, neither polymerase activity is temperature sensitive in vitro.

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