A new method for PCR products cloning - T-A clone technique

Xin Bo Li, Xiao Song Zhao, De Zhi Tian, Yi Chun Zhu, Tai Yao

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


A new method for PCR products cloning - T-A cloning technique is introduced. pBluescript SK (+) plasmid was extracted by modified alkali lysis method. A blunt ends was generated using restriction enzyme EcoRV , then T-A cloning vector was prepared in the presence of Taq DNA polymerase and dTTP. β-actin cDNA fragment was cloned into the above T-A cloning vector. After the recombinant plasmids were digested by EcoR I and HindIII, an expected size of β-actin cDNA fragment was obtained.

Original languageEnglish (US)
Pages (from-to)189
Number of pages1
JournalProgress in Biochemistry and Biophysics
Issue number2
StatePublished - 1999
Externally publishedYes


  • PCR products
  • T-A cloning vector
  • β-actin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry


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