A novel in vitro assay for mouse IgE

Tony J. Hall; Marvin B. Rittenberg

doi:10.1016/0022-1759(85)90016-X

A novel in vitro assay for mouse IgE

Tony J. Hall, Marvin B. Rittenberg

Molecular Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A simple and sensitive assay for screening mouse IgE-producing hybridomas is described. Rat basophil leukemia (RBL) cells (either live or glutaraldehyde fixed) were sensitized by mouse monoclonal IgE and then rosetted with antigen-coated sheep red blood cells. The assay is established using a mouse monoclonal IgE anti-DNP and was able to detect as little as 5 ng/ml IgE. The rosette assay is also a useful technique for studying the binding of mouse IgG monoclonal (MC) antibodies to RBL cells.

Original language	English (US)
Pages (from-to)	145-154
Number of pages	10
Journal	Journal of Immunological Methods
Volume	80
Issue number	2
DOIs	https://doi.org/10.1016/0022-1759(85)90016-X
State	Published - Jun 25 1985

Keywords

IgE and IgG Fc receptors
hybridoma screening
murine IgE and IgG
rat basophil leukemia

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(85)90016-X

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title = "A novel in vitro assay for mouse IgE",

abstract = "A simple and sensitive assay for screening mouse IgE-producing hybridomas is described. Rat basophil leukemia (RBL) cells (either live or glutaraldehyde fixed) were sensitized by mouse monoclonal IgE and then rosetted with antigen-coated sheep red blood cells. The assay is established using a mouse monoclonal IgE anti-DNP and was able to detect as little as 5 ng/ml IgE. The rosette assay is also a useful technique for studying the binding of mouse IgG monoclonal (MC) antibodies to RBL cells.",

keywords = "IgE and IgG Fc receptors, hybridoma screening, murine IgE and IgG, rat basophil leukemia",

author = "Hall, {Tony J.} and Rittenberg, {Marvin B.}",

note = "Funding Information: This work was supported in part by NIH Grant A\[ 14985, ACS Grant IM-172 and Basic Research Grant 1-633 from the March of Dimes Birth Defects Foundation.",

year = "1985",

month = jun,

day = "25",

doi = "10.1016/0022-1759(85)90016-X",

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pages = "145--154",

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T1 - A novel in vitro assay for mouse IgE

AU - Hall, Tony J.

AU - Rittenberg, Marvin B.

N1 - Funding Information: This work was supported in part by NIH Grant A\[ 14985, ACS Grant IM-172 and Basic Research Grant 1-633 from the March of Dimes Birth Defects Foundation.

PY - 1985/6/25

Y1 - 1985/6/25

N2 - A simple and sensitive assay for screening mouse IgE-producing hybridomas is described. Rat basophil leukemia (RBL) cells (either live or glutaraldehyde fixed) were sensitized by mouse monoclonal IgE and then rosetted with antigen-coated sheep red blood cells. The assay is established using a mouse monoclonal IgE anti-DNP and was able to detect as little as 5 ng/ml IgE. The rosette assay is also a useful technique for studying the binding of mouse IgG monoclonal (MC) antibodies to RBL cells.

AB - A simple and sensitive assay for screening mouse IgE-producing hybridomas is described. Rat basophil leukemia (RBL) cells (either live or glutaraldehyde fixed) were sensitized by mouse monoclonal IgE and then rosetted with antigen-coated sheep red blood cells. The assay is established using a mouse monoclonal IgE anti-DNP and was able to detect as little as 5 ng/ml IgE. The rosette assay is also a useful technique for studying the binding of mouse IgG monoclonal (MC) antibodies to RBL cells.

KW - IgE and IgG Fc receptors

KW - hybridoma screening

KW - murine IgE and IgG

KW - rat basophil leukemia

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JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

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ER -

A novel in vitro assay for mouse IgE

Abstract

Keywords

ASJC Scopus subject areas

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