A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

Luz Toribio; Sukwan Handali; Yazmin Marin; Erika Perez; Yesenia Castillo; Javier A. Bustos; Seth E. O’Neal; Hector H. Garcia

doi:10.4269/ajtmh.22-0598

A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

Luz Toribio, Sukwan Handali, Yazmin Marin, Erika Perez, Yesenia Castillo, Javier A. Bustos, Seth E. O’Neal, Hector H. Garcia

School Of Public Health

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.

Original language	English (US)
Pages (from-to)	578-580
Number of pages	3
Journal	American Journal of Tropical Medicine and Hygiene
Volume	108
Issue number	3
DOIs	https://doi.org/10.4269/ajtmh.22-0598
State	Published - Mar 2023

ASJC Scopus subject areas

Parasitology
Infectious Diseases
Virology

Access to Document

10.4269/ajtmh.22-0598

Cite this

@article{40d02a0000974315a07328b9139d6009,

title = "A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples",

abstract = "We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.",

author = "Luz Toribio and Sukwan Handali and Yazmin Marin and Erika Perez and Yesenia Castillo and Bustos, {Javier A.} and O{\textquoteright}Neal, {Seth E.} and Garcia, {Hector H.}",

note = "Publisher Copyright: Copyright {\textcopyright} 2023 The American Society of Tropical Medicine and Hygiene.",

year = "2023",

month = mar,

doi = "10.4269/ajtmh.22-0598",

language = "English (US)",

volume = "108",

pages = "578--580",

journal = "American Journal of Tropical Medicine and Hygiene",

issn = "0002-9637",

publisher = "American Society of Tropical Medicine and Hygiene",

number = "3",

}

TY - JOUR

T1 - A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

AU - Toribio, Luz

AU - Handali, Sukwan

AU - Marin, Yazmin

AU - Perez, Erika

AU - Castillo, Yesenia

AU - Bustos, Javier A.

AU - O’Neal, Seth E.

AU - Garcia, Hector H.

PY - 2023/3

Y1 - 2023/3

N2 - We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.

AB - We report a proof-of-concept study using a dipstick assay to detect Taenia solium antigen in urine samples of 30 patients with subarachnoid neurocysticercosis and 10 healthy control subjects. Strips were read in blind by two readers. The assay detected antigen in 29 of 30 cases and was negative in all 10 control samples. Although this study was performed in samples from individuals with subarachnoid neurocysticercosis who likely had high circulating antigen levels, it provides the proof of concept for a functional urine antigen point-of-care assay that detects viable cysts. Such an assay could serve to support a clinical diagnosis of suspect neurocysticercosis or to identify patients at risk of developing severe disease in areas where medical resources are limited, providing evidence to refer these individuals for imaging and specialized care as needed.

UR - http://www.scopus.com/inward/record.url?scp=85149174944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85149174944&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.22-0598

DO - 10.4269/ajtmh.22-0598

M3 - Article

C2 - 36746658

AN - SCOPUS:85149174944

SN - 0002-9637

VL - 108

SP - 578

EP - 580

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

IS - 3

ER -

A Rapid Point-of-Care Assay for Cysticercosis Antigen Detection in Urine Samples

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this