AAF-cmk sensitizes tumor cells to trail-mediated apoptosis

Izabela Młnarczuk; Paweł Mróz; Grazyna Hoser; Dominika Nowis; Łukasz P. Biały; Halina Ziemba; Tomasz Grzela; Wojciech Feleszko; Jacek Malejczyk; Cezary Wójcik; Marek Jakóbisiak; Jakub Goła̧b

doi:10.1016/S0145-2126(03)00122-X

AAF-cmk sensitizes tumor cells to trail-mediated apoptosis

Izabela Młnarczuk, Paweł Mróz, Grazyna Hoser, Dominika Nowis, Łukasz P. Biały, Halina Ziemba, Tomasz Grzela, Wojciech Feleszko, Jacek Malejczyk, Cezary Wójcik, Marek Jakóbisiak, Jakub Goła̧b

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6 Scopus citations

Abstract

TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This cytokine is cytotoxic for a high proportion of tumor cells, but could be also toxic for normal cells. There is a need to find other agents able to potentiate the antitumor effects of this cytokine. In our study, we found that Ala-Ala-Phe-chloromethylketone (AAF-cmk) augmented cytotoxic activity of TRAIL or TNF against human leukemic cells. Flow cytometry studies and electron microscopy revealed that apoptosis was primarily responsible for this potentiation. Altogether, our studies indicate that AAF-cmk might effectively sensitize human leukemia cells to apoptosis induced by TRAIL and TNF.

Original language	English (US)
Pages (from-to)	53-61
Number of pages	9
Journal	Leukemia Research
Volume	28
Issue number	1
DOIs	https://doi.org/10.1016/S0145-2126(03)00122-X
State	Published - Jan 2004

Keywords

AAF-cmk
Apoptosis
Lymphoma
TNF
TRAIL
Tripeptidylpeptidase II

ASJC Scopus subject areas

Hematology
Oncology
Cancer Research

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10.1016/S0145-2126(03)00122-X

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title = "AAF-cmk sensitizes tumor cells to trail-mediated apoptosis",

abstract = "TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This cytokine is cytotoxic for a high proportion of tumor cells, but could be also toxic for normal cells. There is a need to find other agents able to potentiate the antitumor effects of this cytokine. In our study, we found that Ala-Ala-Phe-chloromethylketone (AAF-cmk) augmented cytotoxic activity of TRAIL or TNF against human leukemic cells. Flow cytometry studies and electron microscopy revealed that apoptosis was primarily responsible for this potentiation. Altogether, our studies indicate that AAF-cmk might effectively sensitize human leukemia cells to apoptosis induced by TRAIL and TNF.",

keywords = "AAF-cmk, Apoptosis, Lymphoma, TNF, TRAIL, Tripeptidylpeptidase II",

author = "Izabela M{\l}narczuk and Pawe{\l} Mr{\'o}z and Grazyna Hoser and Dominika Nowis and Bia{\l}y, {{\L}ukasz P.} and Halina Ziemba and Tomasz Grzela and Wojciech Feleszko and Jacek Malejczyk and Cezary W{\'o}jcik and Marek Jak{\'o}bisiak and Jakub Go{\l}{\c a}b",

note = "Funding Information: This work has been supported by KBN Grant 4 P05A 084 16 to CW, by the Medical University Grant 01-1M15-NS2-2002 to IM and by the CMKP grant 501-2-1-03-48/02 to GH. ",

year = "2004",

month = jan,

doi = "10.1016/S0145-2126(03)00122-X",

language = "English (US)",

volume = "28",

pages = "53--61",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Elsevier Limited",

number = "1",

}

TY - JOUR

T1 - AAF-cmk sensitizes tumor cells to trail-mediated apoptosis

AU - Młnarczuk, Izabela

AU - Mróz, Paweł

AU - Hoser, Grazyna

AU - Nowis, Dominika

AU - Biały, Łukasz P.

AU - Ziemba, Halina

AU - Grzela, Tomasz

AU - Feleszko, Wojciech

AU - Malejczyk, Jacek

AU - Wójcik, Cezary

AU - Jakóbisiak, Marek

AU - Goła̧b, Jakub

N1 - Funding Information: This work has been supported by KBN Grant 4 P05A 084 16 to CW, by the Medical University Grant 01-1M15-NS2-2002 to IM and by the CMKP grant 501-2-1-03-48/02 to GH.

PY - 2004/1

Y1 - 2004/1

N2 - TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This cytokine is cytotoxic for a high proportion of tumor cells, but could be also toxic for normal cells. There is a need to find other agents able to potentiate the antitumor effects of this cytokine. In our study, we found that Ala-Ala-Phe-chloromethylketone (AAF-cmk) augmented cytotoxic activity of TRAIL or TNF against human leukemic cells. Flow cytometry studies and electron microscopy revealed that apoptosis was primarily responsible for this potentiation. Altogether, our studies indicate that AAF-cmk might effectively sensitize human leukemia cells to apoptosis induced by TRAIL and TNF.

AB - TRAIL is a member of the tumor necrosis factor (TNF) superfamily. This cytokine is cytotoxic for a high proportion of tumor cells, but could be also toxic for normal cells. There is a need to find other agents able to potentiate the antitumor effects of this cytokine. In our study, we found that Ala-Ala-Phe-chloromethylketone (AAF-cmk) augmented cytotoxic activity of TRAIL or TNF against human leukemic cells. Flow cytometry studies and electron microscopy revealed that apoptosis was primarily responsible for this potentiation. Altogether, our studies indicate that AAF-cmk might effectively sensitize human leukemia cells to apoptosis induced by TRAIL and TNF.

KW - AAF-cmk

KW - Apoptosis

KW - Lymphoma

KW - TNF

KW - TRAIL

KW - Tripeptidylpeptidase II

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U2 - 10.1016/S0145-2126(03)00122-X

DO - 10.1016/S0145-2126(03)00122-X

M3 - Article

C2 - 14630081

AN - SCOPUS:10744230904

SN - 0145-2126

VL - 28

SP - 53

EP - 61

JO - Leukemia Research

JF - Leukemia Research

IS - 1

ER -

AAF-cmk sensitizes tumor cells to trail-mediated apoptosis

Abstract

Keywords

ASJC Scopus subject areas

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