TY - JOUR
T1 - Abnormal distribution of collagen type IV in extrahepatic bile duct carcinoma
AU - Chen, Yongxin
AU - Sasatomi, Eizaburo
AU - Satoh, Toshimi
AU - Miyazaki, Kohji
AU - Tokunaga, Osamu
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 2000
Y1 - 2000
N2 - The present study investigated the pathogenesis of desmoplastic stroma formation, which is characteristic of most bile duct carcinomas and other scirrhous carcinomas. Using Immunohistochemical analysis, the expression of collagen types I and IV, laminin and TGF-β1 was examined in human extrahepatic bile duct carcinoma and compared with gastric and colon carcinoma. In addition to delineating the basement membranes of carcinoma nests and blood vessels, collagen type IV was present along the thick bundles of collagenous fibers in the stroma of extrahepatic bile duct carcinoma and scirrhous gastric carcinoma. The Immunoreactivity of collagen type IV was strong in the adjacent or surrounding interstitium of tumor cell nests, but was absent or weak in older, more central portions of the tumor that contained sclerotic collagen. In situ hybridization demonstrated active expression of collagen α1(IV) mRNA in extrahepatic bile duct carcinoma and scirrhous gastric carcinoma cells. These results suggest that, although collagen type IV is typically a component of the basement membrane, it is expressed in the interstitial stroma of extrahepatic bile duct carcinoma and scirrhous gastric carcinoma where it may play a role in desmoplastic stroma formation.
AB - The present study investigated the pathogenesis of desmoplastic stroma formation, which is characteristic of most bile duct carcinomas and other scirrhous carcinomas. Using Immunohistochemical analysis, the expression of collagen types I and IV, laminin and TGF-β1 was examined in human extrahepatic bile duct carcinoma and compared with gastric and colon carcinoma. In addition to delineating the basement membranes of carcinoma nests and blood vessels, collagen type IV was present along the thick bundles of collagenous fibers in the stroma of extrahepatic bile duct carcinoma and scirrhous gastric carcinoma. The Immunoreactivity of collagen type IV was strong in the adjacent or surrounding interstitium of tumor cell nests, but was absent or weak in older, more central portions of the tumor that contained sclerotic collagen. In situ hybridization demonstrated active expression of collagen α1(IV) mRNA in extrahepatic bile duct carcinoma and scirrhous gastric carcinoma cells. These results suggest that, although collagen type IV is typically a component of the basement membrane, it is expressed in the interstitial stroma of extrahepatic bile duct carcinoma and scirrhous gastric carcinoma where it may play a role in desmoplastic stroma formation.
KW - Bile duct carcinoma
KW - Collagen type IV
KW - Extracellular matrix
KW - In situ hybridization
KW - Scirrhous carcinoma
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U2 - 10.1046/j.1440-1827.2000.01150.x
DO - 10.1046/j.1440-1827.2000.01150.x
M3 - Article
C2 - 11107064
AN - SCOPUS:0033636856
SN - 1320-5463
VL - 50
SP - 884
EP - 890
JO - Pathology International
JF - Pathology International
IS - 11
ER -