Absence of immunoreactive cytokines in supernatants of individual preimplantation human embryos

D. B. Seifer; R. Romero; D. Berlinsky; R. V. Haning

doi:10.1111/j.1600-0897.1993.tb00610.x

Absence of immunoreactive cytokines in supernatants of individual preimplantation human embryos

D. B. Seifer, R. Romero, D. Berlinsky, R. V. Haning

Obstetrics and Gynecology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.

Original language	English (US)
Pages (from-to)	105-107
Number of pages	3
Journal	American Journal of Reproductive Immunology
Volume	30
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1600-0897.1993.tb00610.x
State	Published - 1993

Keywords

Cytokines
ELISA
human preimplantation embryos

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Reproductive Medicine
Obstetrics and Gynecology

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10.1111/j.1600-0897.1993.tb00610.x

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abstract = "PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.",

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T1 - Absence of immunoreactive cytokines in supernatants of individual preimplantation human embryos

AU - Seifer, D. B.

AU - Romero, R.

AU - Berlinsky, D.

AU - Haning, R. V.

PY - 1993

Y1 - 1993

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AB - PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme-linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL-1 alpha, IL-1 beta, IL-6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.

KW - Cytokines

KW - ELISA

KW - human preimplantation embryos

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Absence of immunoreactive cytokines in supernatants of individual preimplantation human embryos

Abstract

Keywords

ASJC Scopus subject areas

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