Activation by serotonin of starfish eggs expressing the rat serotonin 1c receptor

Fraser Shilling, Gail Mandel, Laurinda A. Jaffe

Research output: Contribution to journalArticlepeer-review

46 Scopus citations


Starfish oocytes were injected with mRNA for the serotonin 1c receptor or with rat brain poly A+ mRNA, incubated to allow expression of the membrane protein, then matured to eggs by addition of 1-methyladenine. Applying serotonin to these eggs caused cortical granule exocytosis like that occurring at fertilization. Because the serotonin 1c receptor specifically activates a G-protein, these results provide support for the hypothesis that sperm activate eggs by way of a receptor-G-protein interaction. The starfish oocyte may be a generally useful system for expression of exogenous mRNA for membrane proteins.

Original languageEnglish (US)
Pages (from-to)465-469
Number of pages5
JournalMolecular biology of the cell
Issue number6
StatePublished - May 1990
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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