@article{3d91368ebcf245c7a832314c2728b54c,
title = "Adjacent Cell Marker Lateral Spillover Compensation and Reinforcement for Multiplexed Images",
abstract = "Multiplex imaging technologies are now routinely capable of measuring more than 40 antibody-labeled parameters in single cells. However, lateral spillage of signals in densely packed tissues presents an obstacle to the assignment of high-dimensional spatial features to individual cells for accurate cell-type annotation. We devised a method to correct for lateral spillage of cell surface markers between adjacent cells termed REinforcement Dynamic Spillover EliminAtion (REDSEA). The use of REDSEA decreased contaminating signals from neighboring cells. It improved the recovery of marker signals across both isotopic (i.e., Multiplexed Ion Beam Imaging) and immunofluorescent (i.e., Cyclic Immunofluorescence) multiplexed images resulting in a marked improvement in cell-type classification.",
keywords = "cell annotation, image correction, multiplexed tissue imaging, signal spillover, single-cell biology, spatial proteomics",
author = "Yunhao Bai and Bokai Zhu and Xavier Rovira-Clave and Han Chen and Maxim Markovic and Chan, {Chi Ngai} and Su, {Tung Hung} and McIlwain, {David R.} and Estes, {Jacob D.} and Leeat Keren and Nolan, {Garry P.} and Sizun Jiang",
note = "Funding Information: SJ is supported by the Leukemia & Lymphoma Society Career Development Program. BZ is supported by a Stanford Graduate Fellowship. This work was funded in part by grants from the National Cancer Institute, National Institutes of Health, Task Order No. HHSN261100039 under Contract No. HHSN261201500003I (GN) and U2CCA233195 (GN), the National Institutes of Health R01AI149672 (GN and JE), the Bill & Melinda Gates Foundation INV-002704 (GN and JE), OPP1113682 (GN), and COVID-19 Pilot Award (SJ, DM, GN), the Fast Grant Funding for COVID-19 Science (GN), the Food and Drug Administration HHSF223201610018C (GN), the Parker Institute for Cancer Immunotherapy (GN), and the Rachford and Carlota A. Harris Endowed Professorship (GN). This article reflects the views of the authors and should not be construed as representing the views or policies of the FDA, NIH, BMGF or other institutions who provided funding. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Bai, Zhu, Rovira-Clave, Chen, Markovic, Chan, Su, McIlwain, Estes, Keren, Nolan and Jiang.",
year = "2021",
month = jul,
day = "5",
doi = "10.3389/fimmu.2021.652631",
language = "English (US)",
volume = "12",
journal = "Frontiers in immunology",
issn = "1664-3224",
publisher = "Frontiers Media S. A.",
}