@article{062f30da890f428d8c33b8aeb1a3a9f1,
title = "An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC89) from embryonic fibroblasts",
abstract = "We generated a rhesus macaque induced pluripotent stem cell (riPSC) line, riPSC89, from rhesus embryonic fibroblasts (REFs). Fibroblasts were expanded from the skin of a rhesus macaque embryo at embryonic day 47. REFs and riPSCs had a normal male (42, XY) karyotype. The riPSC89 line was positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4. Pluripotency was demonstrated through the generation of teratomas using transplantation into immunocompromised mice. The riPSC89 line may be a useful non-human primate resource to uncover developmental origins of disease, or used as a basic model to understand lineage specification in the primate embryo.",
author = "Enrique Sosa and Rachel Kim and Rojas, {Ernesto J.} and Linzi Hosohama and Hennebold, {Jon D.} and Orwig, {Kyle E.} and Clark, {Amander T.}",
note = "Funding Information: We would like to thank Dr. Saran Karumbayaram for providing guidance in the culture and expansion of the primate fibroblasts. We would also like to thank Steven Dowdy for depositing the T7-VEE-OKS-iG plasmid (Addgene). This project was funded by NIH grant P01HD075795 (KEO and ATC). Non human primate time mated breeding was supported by NIH OD P51OD011092 (JDH). EJR was supported by the NIH initative to maximize student diversity in science (NIH GM55052 ) and NSF HRD00603239 . Publisher Copyright: {\textcopyright} 2016 Helmholtz Zentrum M{\"u}nchen",
year = "2016",
month = sep,
doi = "10.1016/j.scr.2016.09.015",
language = "English (US)",
volume = "17",
pages = "444--447",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
number = "2",
}