Analysis of Serum Anti-Zika Virus Antibodies by Focus Reduction Neutralization Test

Jessica L. Smith, Alec J. Hirsch

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


Zika virus (ZIKV) is a newly emerged mosquito-borne flavivirus that has been associated with birth defects of babies born to ZIKV-infected mothers. Neutralization activity of serum derived from ZIKV infected and vaccinated individuals is a critical component for characterizing immune response to infection and vaccine efficacy. This protocol describes a modified plaque reduction neutralization 50 (PRNT50) assay that includes an immunostaining step to improve reproducibility and throughput of the assay.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages8
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Antibody
  • Flavivirus
  • Focus reduction neutralization
  • Immunostain
  • Plaque reduction neutralization
  • Zika virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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