TY - CHAP
T1 - Anatomical, physiological, and behavioral analysis of rodent vision
AU - Jeffrey, Brett G.
AU - McGill, Trevor J.
AU - Haley, Tammie L.
AU - Morgans, Catherine W.
AU - Duvoisin, Robert M.
PY - 2011
Y1 - 2011
N2 - This chapter provides protocols for the study of rodent vision. An advantage of the visual system is that the physiological and behavioral response to the natural stimulus, light, can be measured. Moreover, the anatomy and circuitry of the system have been the subject of much research. Here, we describe our protocols for the analysis of the distribution of neurotransmitter receptors and signaling molecules in the retina by immunohistochemistry. We also explain in detail how we record the electroretinogram from mice, and we review two behavioral tests of rodent vision. One, the virtual optomotor test, makes use of the optokinetic nystagmus reflex, and thus is simple to perform and does not require training. The other, the visual water maze, is more demanding but provides a true quantitative readout of vision performance.
AB - This chapter provides protocols for the study of rodent vision. An advantage of the visual system is that the physiological and behavioral response to the natural stimulus, light, can be measured. Moreover, the anatomy and circuitry of the system have been the subject of much research. Here, we describe our protocols for the analysis of the distribution of neurotransmitter receptors and signaling molecules in the retina by immunohistochemistry. We also explain in detail how we record the electroretinogram from mice, and we review two behavioral tests of rodent vision. One, the virtual optomotor test, makes use of the optokinetic nystagmus reflex, and thus is simple to perform and does not require training. The other, the visual water maze, is more demanding but provides a true quantitative readout of vision performance.
KW - Mouse
KW - Virtual Optomotor System
KW - acuity
KW - confocal light microscopy
KW - contrast sensitivity
KW - electroretinogram
KW - immunohistochemistry
KW - retina
KW - visual water maze
UR - http://www.scopus.com/inward/record.url?scp=77957926332&partnerID=8YFLogxK
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U2 - 10.1007/978-1-60761-883-6_2
DO - 10.1007/978-1-60761-883-6_2
M3 - Chapter
AN - SCOPUS:77957926332
SN - 9781607618829
T3 - Neuromethods
SP - 29
EP - 54
BT - Animal Models of Behavioral Analysis
A2 - Raber, Jacob
ER -