Abstract
3α-Androstanediol glucuronide (Adiol G) is a major metabolite of dihydrotestosterone (DHT). Adiol G actually represents 2 different compounds, since the glucuronide can be conjugated at the 3-carbon position (Adiol 3-G) or at the 17-carbon position (Adiol 17-G). To determine which glucuronide represents the predominant physiological DHT metabolite and which isomer is the major circulating form, we developed a RIA to directly measure Adiol 3-G in serum extracts. In 10 normal men, mean serum Adiol 3-G and total Adiol G levels were 4.44 ± 0.49 (±se>) nmol/L (208 ± 23 ng/dL) and 27.9 ± 2.8 nmol/L (1310 ± 129 ng/dL), respectively (13.9 ± 3.0% of Adiol G was Adiol 3-G). In 10 normal women sampled during the early follicular phase, mean serum Adiol 3-G and total Adiol G levels were 2.64 ± 0.64 nmol/L (124 ± 30 ng/dL) and 14.9 ± 1.5 nmol/L (697 ± 69 ng/dL), respectively (17.4 ± 3.6% of Adiol G was Adiol 3-G). In 4 normal men infused for 8 h with tritiated DHT, 17.4 ± 3.4% of the resulting tritiated Adiol G was Adiol 3-G. These results indicate that Adiol 17-G is the predominant circulating form of Adiol G in normal men and women and that it is also the major Adiol G isomer derived from DHT.
Original language | English (US) |
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Pages (from-to) | 212-216 |
Number of pages | 5 |
Journal | Journal of Clinical Endocrinology and Metabolism |
Volume | 66 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1988 |
Externally published | Yes |
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Biochemistry
- Endocrinology
- Clinical Biochemistry
- Biochemistry, medical