Angiotensin II receptor subtypes in the rat brain

Brian P. Rowe; Kevin L. Grove; David L. Saylor; Robert C. Speth

doi:10.1016/0014-2999(90)90457-H

Angiotensin II receptor subtypes in the rat brain

Brian P. Rowe, Kevin L. Grove, David L. Saylor, Robert C. Speth

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

The non-peptide angiotensin II (AII) receptor subtype selective antagonist, DuP 753, was used to characterize AII receptor binding sites in the rat brain. DuP 753 competed for specific ¹²⁵I-[Sar¹, Ile⁸]AII (¹²⁵I-SIAII) binding in many brain nuclei (IC₅₀ = 20-30 nM), but was a weak competitor at remaining sites (IC₅₀ > 10^-4 M). DuP 753 sensitive binding sites (designated AII_α subtype) correspond with areas where binding is inhibited by sulfhydryl reducing agents, whereas DuP 753 insensitive sites (AII_β) correspond with areas where binding is not inhibited by sulfhydryl reducing agents.

Original language	English (US)
Pages (from-to)	339-342
Number of pages	4
Journal	European Journal of Pharmacology
Volume	186
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-2999(90)90457-H
State	Published - Sep 21 1990
Externally published	Yes

Keywords

(Rat)
(Receptor subtypes)
Angiotensin II receptors
Brain
DuP 753
Receptor autoradiography

ASJC Scopus subject areas

Pharmacology

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10.1016/0014-2999(90)90457-H

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title = "Angiotensin II receptor subtypes in the rat brain",

abstract = "The non-peptide angiotensin II (AII) receptor subtype selective antagonist, DuP 753, was used to characterize AII receptor binding sites in the rat brain. DuP 753 competed for specific 125I-[Sar1, Ile8]AII (125I-SIAII) binding in many brain nuclei (IC50 = 20-30 nM), but was a weak competitor at remaining sites (IC50 > 10-4 M). DuP 753 sensitive binding sites (designated AIIα subtype) correspond with areas where binding is inhibited by sulfhydryl reducing agents, whereas DuP 753 insensitive sites (AIIβ) correspond with areas where binding is not inhibited by sulfhydryl reducing agents.",

keywords = "(Rat), (Receptor subtypes), Angiotensin II receptors, Brain, DuP 753, Receptor autoradiography",

author = "Rowe, {Brian P.} and Grove, {Kevin L.} and Saylor, {David L.} and Speth, {Robert C.}",

note = "Funding Information: We thank Dr. Pieter Timmermans of DuPont for supplying DuP 753 and Teresa Lloyd-Saylor for providing technical assistance. The study was supported by The American Heart Association, Tennessee Affiliate and NIH (NS-24388).",

year = "1990",

month = sep,

day = "21",

doi = "10.1016/0014-2999(90)90457-H",

language = "English (US)",

volume = "186",

pages = "339--342",

journal = "European Journal of Pharmacology",

issn = "0014-2999",

publisher = "Elsevier",

number = "2-3",

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TY - JOUR

T1 - Angiotensin II receptor subtypes in the rat brain

AU - Rowe, Brian P.

AU - Grove, Kevin L.

AU - Saylor, David L.

AU - Speth, Robert C.

N1 - Funding Information: We thank Dr. Pieter Timmermans of DuPont for supplying DuP 753 and Teresa Lloyd-Saylor for providing technical assistance. The study was supported by The American Heart Association, Tennessee Affiliate and NIH (NS-24388).

PY - 1990/9/21

Y1 - 1990/9/21

N2 - The non-peptide angiotensin II (AII) receptor subtype selective antagonist, DuP 753, was used to characterize AII receptor binding sites in the rat brain. DuP 753 competed for specific 125I-[Sar1, Ile8]AII (125I-SIAII) binding in many brain nuclei (IC50 = 20-30 nM), but was a weak competitor at remaining sites (IC50 > 10-4 M). DuP 753 sensitive binding sites (designated AIIα subtype) correspond with areas where binding is inhibited by sulfhydryl reducing agents, whereas DuP 753 insensitive sites (AIIβ) correspond with areas where binding is not inhibited by sulfhydryl reducing agents.

AB - The non-peptide angiotensin II (AII) receptor subtype selective antagonist, DuP 753, was used to characterize AII receptor binding sites in the rat brain. DuP 753 competed for specific 125I-[Sar1, Ile8]AII (125I-SIAII) binding in many brain nuclei (IC50 = 20-30 nM), but was a weak competitor at remaining sites (IC50 > 10-4 M). DuP 753 sensitive binding sites (designated AIIα subtype) correspond with areas where binding is inhibited by sulfhydryl reducing agents, whereas DuP 753 insensitive sites (AIIβ) correspond with areas where binding is not inhibited by sulfhydryl reducing agents.

KW - (Rat)

KW - (Receptor subtypes)

KW - Angiotensin II receptors

KW - Brain

KW - DuP 753

KW - Receptor autoradiography

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U2 - 10.1016/0014-2999(90)90457-H

DO - 10.1016/0014-2999(90)90457-H

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EP - 342

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

IS - 2-3

ER -

Angiotensin II receptor subtypes in the rat brain

Abstract

Keywords

ASJC Scopus subject areas

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