Antesera to the ganglioside GM1 do not have anti-myelin or anti-axon activities in vitro

Dennis N. Bourdette; Bernard Zalc; Nicole Baumann; Frederick J. Seil

doi:10.1016/0006-8993(89)91493-5

Antesera to the ganglioside GM₁ do not have anti-myelin or anti-axon activities in vitro

Dennis N. Bourdette
, Bernard Zalc
, Nicole Baumann
, Frederick J. Seil

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Four antisera to the ganglioside GM₁ were tested effects on myelin and axons when applied to mouse spinal cord-dorsal root ganglia explant cultures. None of the antisera to GM₁ caused myelination inhibition or demyelination, while an antiserum tto galactocerebroside caused both. Antisera to GM₁ did not axonal outgrowth or destroy mature outgrowth zone axons, while an anteserum to a rat brain axolemma-enriched fraction did both. These results suggest that antibodies to GM₁ do not have significant anti-myelin or anti-axon activity.

Original language	English (US)
Pages (from-to)	175-180
Number of pages	6
Journal	Brain Research
Volume	478
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(89)91493-5
State	Published - Jan 23 1989
Externally published	Yes

Funding

This work was supported by the Veterans Administration (DNB and FJS) and by a grant from the Association de Recherche sur la Scl6rose en Plaque (BZ). We thank Dr. George DeVries of the Medical College of Virginia for supplying the axolemma-enriched fraction antiserun~,. We thank Daniel Austin for helping prepare the figure and Lynn Riley and Karin Coffee for their secretarial assistance.

Funders
Association de Recherche sur la Scl6rose en Plaque
Veterans Administration

Keywords

Anti-axon antibody
Demyelination
Ganglioside antibody
Myelination inhibition
Tissue culture

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Access to Document

10.1016/0006-8993(89)91493-5

Cite this

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title = "Antesera to the ganglioside GM1 do not have anti-myelin or anti-axon activities in vitro",

abstract = "Four antisera to the ganglioside GM1 were tested effects on myelin and axons when applied to mouse spinal cord-dorsal root ganglia explant cultures. None of the antisera to GM1 caused myelination inhibition or demyelination, while an antiserum tto galactocerebroside caused both. Antisera to GM1 did not axonal outgrowth or destroy mature outgrowth zone axons, while an anteserum to a rat brain axolemma-enriched fraction did both. These results suggest that antibodies to GM1 do not have significant anti-myelin or anti-axon activity.",

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note = "Funding Information: This work was supported by the Veterans Administration (DNB and FJS) and by a grant from the Association de Recherche sur la Scl6rose en Plaque (BZ). We thank Dr. George DeVries of the Medical College of Virginia for supplying the axolemma-enriched fraction antiserun\textasciitilde{},. We thank Daniel Austin for helping prepare the figure and Lynn Riley and Karin Coffee for their secretarial assistance.",

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AU - Bourdette, Dennis N.

AU - Zalc, Bernard

AU - Baumann, Nicole

AU - Seil, Frederick J.

N1 - Funding Information: This work was supported by the Veterans Administration (DNB and FJS) and by a grant from the Association de Recherche sur la Scl6rose en Plaque (BZ). We thank Dr. George DeVries of the Medical College of Virginia for supplying the axolemma-enriched fraction antiserun~,. We thank Daniel Austin for helping prepare the figure and Lynn Riley and Karin Coffee for their secretarial assistance.

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N2 - Four antisera to the ganglioside GM1 were tested effects on myelin and axons when applied to mouse spinal cord-dorsal root ganglia explant cultures. None of the antisera to GM1 caused myelination inhibition or demyelination, while an antiserum tto galactocerebroside caused both. Antisera to GM1 did not axonal outgrowth or destroy mature outgrowth zone axons, while an anteserum to a rat brain axolemma-enriched fraction did both. These results suggest that antibodies to GM1 do not have significant anti-myelin or anti-axon activity.

AB - Four antisera to the ganglioside GM1 were tested effects on myelin and axons when applied to mouse spinal cord-dorsal root ganglia explant cultures. None of the antisera to GM1 caused myelination inhibition or demyelination, while an antiserum tto galactocerebroside caused both. Antisera to GM1 did not axonal outgrowth or destroy mature outgrowth zone axons, while an anteserum to a rat brain axolemma-enriched fraction did both. These results suggest that antibodies to GM1 do not have significant anti-myelin or anti-axon activity.

KW - Anti-axon antibody

KW - Demyelination

KW - Ganglioside antibody

KW - Myelination inhibition

KW - Tissue culture

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