TY - JOUR
T1 - Assessing burn wound depth using in vitro nuclear magnetic resonance (NMR)
AU - Koruda, Mark J.
AU - Zimbler, Andrea
AU - Settle, R. Gregg
AU - Jacobs, Danny O.
AU - Rolandelli, Rolando H.
AU - Wolf, Gerald L.
AU - Rombeau, John L.
N1 - Funding Information:
Supported, in part, by funds from the Veterans Administration, PHS Grant NSl6365. We gratefully appreciate the technical assistance of Ms. Patricia Boyle.
PY - 1986/5
Y1 - 1986/5
N2 - There is no accurate noninvasive method for differentiating between partial-thickness and full-thickness cutaneous burn wounds. Full-thickness burns (FTB) result in slower resorption of wound edema than partial thickness burns (PTB). Since proton NMR parameters, particularly the T1 relaxation time, correlate with tissue water content (TWC), the present study determined whether proton NMR could distinguish PTB from FTB. An area of upper dorsum (∼ 15% BSA) of 35 adult rats was immersed in boiling water for either 3 sec (PTB) or 10 sec (FTB). In 10 control rats, the same area was immersed in room temperature water. Rats were sacrificed at either 3 or 48 hr after burn, and skin samples were analyzed to determine spin-lattice (T1) and spin-spin (T2) relaxation times. TWC was then measured gravimetrically by desiccation. Both T1 and T2 times significantly correlated with TWC (T1: r = 0.74, P < 0.0001; T2: r = 0.75; P < 0.0001). Both PTB and FTB resulted in significant elevations of T1, T2, and TWC 3 hr after injury (P > 0.001). At 48 hr postburn the T1, T2, and TWC of the PTB group had decreased to control values (P > 0.05), while all FTB parameters remained significantly elevated as compared to both the control and the 48-hr PTB parameters (P < 0.001). In vitro NMR measurements distinguish PTB from FTB in this rat model within 48 hr. These data provide a basis for investigating in vitro NMR techniques for the noninvasive assessment of burn wound depth.
AB - There is no accurate noninvasive method for differentiating between partial-thickness and full-thickness cutaneous burn wounds. Full-thickness burns (FTB) result in slower resorption of wound edema than partial thickness burns (PTB). Since proton NMR parameters, particularly the T1 relaxation time, correlate with tissue water content (TWC), the present study determined whether proton NMR could distinguish PTB from FTB. An area of upper dorsum (∼ 15% BSA) of 35 adult rats was immersed in boiling water for either 3 sec (PTB) or 10 sec (FTB). In 10 control rats, the same area was immersed in room temperature water. Rats were sacrificed at either 3 or 48 hr after burn, and skin samples were analyzed to determine spin-lattice (T1) and spin-spin (T2) relaxation times. TWC was then measured gravimetrically by desiccation. Both T1 and T2 times significantly correlated with TWC (T1: r = 0.74, P < 0.0001; T2: r = 0.75; P < 0.0001). Both PTB and FTB resulted in significant elevations of T1, T2, and TWC 3 hr after injury (P > 0.001). At 48 hr postburn the T1, T2, and TWC of the PTB group had decreased to control values (P > 0.05), while all FTB parameters remained significantly elevated as compared to both the control and the 48-hr PTB parameters (P < 0.001). In vitro NMR measurements distinguish PTB from FTB in this rat model within 48 hr. These data provide a basis for investigating in vitro NMR techniques for the noninvasive assessment of burn wound depth.
UR - http://www.scopus.com/inward/record.url?scp=0022475401&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022475401&partnerID=8YFLogxK
U2 - 10.1016/0022-4804(86)90218-0
DO - 10.1016/0022-4804(86)90218-0
M3 - Article
C2 - 3736031
AN - SCOPUS:0022475401
SN - 0022-4804
VL - 40
SP - 475
EP - 481
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 5
ER -