Autoantibody profile in eosinophilic granulomatosis and polyangiitis: Predominance of anti-alpha-enolase antibodies

K. Laskari, B. Hellmich, G. Adamus, E. Csernok

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Objective. To evaluate the autoantibody profile in eosinophilic granulomatosis and polyangiitis (EGPA) patients. Methods. 33 EGPA patients were tested for anti-neutrophil cytoplasmic antibodies (ANCA), antinuclear antibodies (ANA), rheumatoid factor (RF), anti-alpha-enolase antibodies, and anti-eosinophil peroxidase (EPO) antibodies. Granulomatosis with polyangiitis (GPA), microscopic polyangiitis (MPA), hypereosinophilic syndrome (HES), rheumatoid arthritis (RA), primary biliary cirrhosis (PBC) patients and healthy subjects were tested as a control group. Results. Anti-alpha-enolase antibodies were positive in 82% of EGPA patients at high titres. Although a high sensitivity was shown for an anti-alpha-enolase antibody titre above 1/100 (82%), the specificity for EGPA remained low (44%) (AUC=0.653, p=0.008). Anti-alpha-enolase antibodies predominated in males with EGPA (p=0.048) and were associated with skin involvement (p=0.040). Most of the EGPA patients positive for anti-alpha enolase antibodies (20 out of 27) had a negative indirect immunofluorescence test (IFT) for ANCA. ANCA were positive in 8 EGPA patients (24%) with a perinuclear pattern in all but one patient. The ANCA-target antigen was myeloperoxidase (MPO) and/or alpha-enolase. A usually fine-speckled ANA pattern was observed in 42% of the EGPA patients. RF was positive in 1 (6%) of the 18 EGPA patients tested. There was no association between the presence and levels of autoantibodies and EGPA disease activity. None of the patients and controls was positive for anti-EPO antibodies. Conclusion. Alpha-enolase may be a target of autoimmunity in EGPA patients and shows usually negative ANCA IFT results.

Original languageEnglish (US)
Pages (from-to)S83-S87
JournalClinical and experimental rheumatology
Issue number2
StatePublished - 2021


  • Anti-alpha-enolase antibodies
  • Anti-neutrophil cytoplasmic antibodies (ANCA)
  • Eosinophilic granulomatosis and polyangiitis (EGPA)

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Autoantibody profile in eosinophilic granulomatosis and polyangiitis: Predominance of anti-alpha-enolase antibodies'. Together they form a unique fingerprint.

Cite this