TY - JOUR
T1 - Autoimmune ovarian disease induced by immunization with zona pellucida (ZP3) peptide.
AU - Tung, Kenneth
AU - Agersborg, Sally
AU - Bagavant, Harini
AU - Garza, Kristine
AU - Wei, Kevin
PY - 2002/8
Y1 - 2002/8
N2 - Autoimmune ovarian disease is a known cause of human premature ovarian failure. An experimental murine model can be created by immunization with a peptide of ZP3, an ovary-specific glycoprotein. Mice injected with the ZP3 peptide develop histological evidence of ovarian inflammation (oophoritis), as well as antibody to the zona pellucida and T cell responses to the peptide. This unit describes the immunologic properties of the ZP3 peptides, the method of active autoimmune oophoritis induction, and the evaluation and semiquantitation of ovarian pathology. Surgical manipulations of the ovaries have been instrumental in the novel application of this autoimmune model; thus the most useful operations are also detailed. Surgical procedures are included for adult oophrectomy, neonatal ovariectomy, and implantation of ovarian grafts into mice without endogenous ovaries.
AB - Autoimmune ovarian disease is a known cause of human premature ovarian failure. An experimental murine model can be created by immunization with a peptide of ZP3, an ovary-specific glycoprotein. Mice injected with the ZP3 peptide develop histological evidence of ovarian inflammation (oophoritis), as well as antibody to the zona pellucida and T cell responses to the peptide. This unit describes the immunologic properties of the ZP3 peptides, the method of active autoimmune oophoritis induction, and the evaluation and semiquantitation of ovarian pathology. Surgical manipulations of the ovaries have been instrumental in the novel application of this autoimmune model; thus the most useful operations are also detailed. Surgical procedures are included for adult oophrectomy, neonatal ovariectomy, and implantation of ovarian grafts into mice without endogenous ovaries.
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M3 - Article
C2 - 18432873
AN - SCOPUS:43549083687
SN - 1934-3671
VL - Chapter 15
SP - Unit 15.17
JO - Current protocols in immunology / edited by John E. Coligan ... [et al.]
JF - Current protocols in immunology / edited by John E. Coligan ... [et al.]
ER -