Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis

D. B. Seifer, M. R. Freeman, A. C. Gardiner, G. A. Hill, A. L. Schneyer, B. C. Vanderhyden

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Objective: To determine if embryos can modulate steroid hormone production by luteinized granulosa cells. Design: Granulosa cells obtained from follicular aspirates were cultured alone or in the presence of a two- pronuclear zygote. The production of E2 and P by these cultures was evaluated by RIA. Setting: In Vitro Fertilization Unit in an academic research environment. Patients: Sixteen women undergoing IVF. Interventions: Standard IVF-ET treatment cycle using leuprolide acetate for pituitary desensitization before hMG or urofollitropin for ovarian stimulation. Main Outcome Measures: Estradiol and P concentration in culture media of luteinized granulosa cells alone or granulosa cells cocultured with a two- pronuclear embryo. Results: Both E2 and P production by luteinized granulosa cells was reduced when cultured in the presence of an embryo. Conclusions: Human embryos secrete a factor that regulates granulosa cell steroidogenesis.

Original languageEnglish (US)
Pages (from-to)425-429
Number of pages5
JournalFertility and sterility
Issue number3
StatePublished - 1996
Externally publishedYes


  • Granulosa cell steroidogenesis
  • autologous granulosa cell coculture
  • embryo development
  • estradiol
  • progesterone

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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