Binding and processing of epidermal growth factor in Panc-I human pancreatic carcinoma cells

Murray Korc; Bruce E. Magun

doi:10.1016/0024-3205(85)90158-4

Binding and processing of epidermal growth factor in Panc-I human pancreatic carcinoma cells

Murray Korc, Bruce E. Magun

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The binding of ¹²⁵I-labeled epidermal growth factor (EGF) was studied in Panc-I human pancreatic carcinoma cells. At 37°C, binding was rapid and associated with marked endocytosis of the ligand. Bound EGF was sequentially converted to a number of more acidic species as follows: pI 4.55 to pI 4.2, to pI 4.35, to pI 4.0. EGF internalization and processing were blocked at 4°C. EGF did not alter cell growth when Panc-I cells were incubated in the presence of 2 to 10% serum. In contrast, when the serum concentration was lowered to 0.1%, EGF significantly enhanced cell replication after 6 days of culture.

Original language	English (US)
Pages (from-to)	1849-1855
Number of pages	7
Journal	Life Sciences
Volume	36
Issue number	19
DOIs	https://doi.org/10.1016/0024-3205(85)90158-4
State	Published - May 13 1985
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(85)90158-4

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title = "Binding and processing of epidermal growth factor in Panc-I human pancreatic carcinoma cells",

abstract = "The binding of 125I-labeled epidermal growth factor (EGF) was studied in Panc-I human pancreatic carcinoma cells. At 37°C, binding was rapid and associated with marked endocytosis of the ligand. Bound EGF was sequentially converted to a number of more acidic species as follows: pI 4.55 to pI 4.2, to pI 4.35, to pI 4.0. EGF internalization and processing were blocked at 4°C. EGF did not alter cell growth when Panc-I cells were incubated in the presence of 2 to 10% serum. In contrast, when the serum concentration was lowered to 0.1%, EGF significantly enhanced cell replication after 6 days of culture.",

author = "Murray Korc and Magun, {Bruce E.}",

note = "Funding Information: CA-23074 (Cancer Center Core Support Grant) from the National Institutes of Health.",

year = "1985",

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doi = "10.1016/0024-3205(85)90158-4",

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T1 - Binding and processing of epidermal growth factor in Panc-I human pancreatic carcinoma cells

AU - Korc, Murray

AU - Magun, Bruce E.

N1 - Funding Information: CA-23074 (Cancer Center Core Support Grant) from the National Institutes of Health.

PY - 1985/5/13

Y1 - 1985/5/13

N2 - The binding of 125I-labeled epidermal growth factor (EGF) was studied in Panc-I human pancreatic carcinoma cells. At 37°C, binding was rapid and associated with marked endocytosis of the ligand. Bound EGF was sequentially converted to a number of more acidic species as follows: pI 4.55 to pI 4.2, to pI 4.35, to pI 4.0. EGF internalization and processing were blocked at 4°C. EGF did not alter cell growth when Panc-I cells were incubated in the presence of 2 to 10% serum. In contrast, when the serum concentration was lowered to 0.1%, EGF significantly enhanced cell replication after 6 days of culture.

AB - The binding of 125I-labeled epidermal growth factor (EGF) was studied in Panc-I human pancreatic carcinoma cells. At 37°C, binding was rapid and associated with marked endocytosis of the ligand. Bound EGF was sequentially converted to a number of more acidic species as follows: pI 4.55 to pI 4.2, to pI 4.35, to pI 4.0. EGF internalization and processing were blocked at 4°C. EGF did not alter cell growth when Panc-I cells were incubated in the presence of 2 to 10% serum. In contrast, when the serum concentration was lowered to 0.1%, EGF significantly enhanced cell replication after 6 days of culture.

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Binding and processing of epidermal growth factor in Panc-I human pancreatic carcinoma cells

Abstract

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