Biochemical and immunological evidence for a calcium pump in chromaffin granules

Steven C. King, Thomas E. Ellenberger, Stanley M. Goldin

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


ATP stimulated the accumulation of 45Ca2+ by chromaffin granule ghosts which contained 5 mM oxalate to trap transported calcium within the lumen. Inasmuch as the ATP-dependent 45Ca2+ transport was resistant to 25 mM ammonium acetate as well as the proton ionophore, carbonylcyanide-m-chlorophenylhydrazone, the chromaffin granule proton translocating ATPase does not provide the energy for this process. Instead, we suggest that chromaffin granules contain a calcium translocating ATPase which catalyzes the 45Ca2+ uptake directly. The observation that chromaffin granules bind to a monoclonal antibody raised against a calcium pump from bovine brain supports this hypothesis.

Original languageEnglish (US)
Pages (from-to)656-663
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - Sep 15 1988
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Biochemical and immunological evidence for a calcium pump in chromaffin granules'. Together they form a unique fingerprint.

Cite this