Bleomycin-induced breakage of closed-circular DNA

James C. Bearden; R. Stephen Lloyd; Charles W. Haidle

doi:10.1016/0006-291X(77)91062-2

Bleomycin-induced breakage of closed-circular DNA

James C. Bearden, R. Stephen Lloyd, Charles W. Haidle

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The amount of breakage induced by bleomycin in closed-circular DNA from bacteriophage PM2 was investigated under various reaction conditions. Analysis of the extent of breakage, by alkaline band centrifugation and agarose gel electrophoresis, yielded quite different results from those previously reported for the breakage of closed-circular SV40 DNA by bleomycin. Compared to these earlier results, we found (a) the reaction required the addition of 2-mercaptoethanol; and (b) the breakage reaction was further stimulated by the addition of 2-mercaptoethanol in the absence of EDTA.

Original language	English (US)
Pages (from-to)	442-448
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	75
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(77)91062-2
State	Published - Mar 21 1977
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(77)91062-2

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title = "Bleomycin-induced breakage of closed-circular DNA",

abstract = "The amount of breakage induced by bleomycin in closed-circular DNA from bacteriophage PM2 was investigated under various reaction conditions. Analysis of the extent of breakage, by alkaline band centrifugation and agarose gel electrophoresis, yielded quite different results from those previously reported for the breakage of closed-circular SV40 DNA by bleomycin. Compared to these earlier results, we found (a) the reaction required the addition of 2-mercaptoethanol; and (b) the breakage reaction was further stimulated by the addition of 2-mercaptoethanol in the absence of EDTA.",

author = "Bearden, {James C.} and Lloyd, {R. Stephen} and Haidle, {Charles W.}",

note = "Funding Information: ACKNOWLEDGEMENTS This investigation was supported, in part, by Public Health Research Grant Number CA-13246, from the National Cancer Institute; Grant G-441, from the Robert A. Welch Foundation; and by a Public Service Postdoctoral Research Fellowship, No. l-F32-CA05029-01, National Cancer Institute, awarded to James Bearden, Jr.",

year = "1977",

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doi = "10.1016/0006-291X(77)91062-2",

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T1 - Bleomycin-induced breakage of closed-circular DNA

AU - Bearden, James C.

AU - Lloyd, R. Stephen

AU - Haidle, Charles W.

N1 - Funding Information: ACKNOWLEDGEMENTS This investigation was supported, in part, by Public Health Research Grant Number CA-13246, from the National Cancer Institute; Grant G-441, from the Robert A. Welch Foundation; and by a Public Service Postdoctoral Research Fellowship, No. l-F32-CA05029-01, National Cancer Institute, awarded to James Bearden, Jr.

PY - 1977/3/21

Y1 - 1977/3/21

N2 - The amount of breakage induced by bleomycin in closed-circular DNA from bacteriophage PM2 was investigated under various reaction conditions. Analysis of the extent of breakage, by alkaline band centrifugation and agarose gel electrophoresis, yielded quite different results from those previously reported for the breakage of closed-circular SV40 DNA by bleomycin. Compared to these earlier results, we found (a) the reaction required the addition of 2-mercaptoethanol; and (b) the breakage reaction was further stimulated by the addition of 2-mercaptoethanol in the absence of EDTA.

AB - The amount of breakage induced by bleomycin in closed-circular DNA from bacteriophage PM2 was investigated under various reaction conditions. Analysis of the extent of breakage, by alkaline band centrifugation and agarose gel electrophoresis, yielded quite different results from those previously reported for the breakage of closed-circular SV40 DNA by bleomycin. Compared to these earlier results, we found (a) the reaction required the addition of 2-mercaptoethanol; and (b) the breakage reaction was further stimulated by the addition of 2-mercaptoethanol in the absence of EDTA.

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Bleomycin-induced breakage of closed-circular DNA

Abstract

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