Brain insulin binding is decreased in Wistar Kyoto rats carrying the 'fa' gene

D. P. Figlewicz; H. Ikeda; T. R. Hunt; L. J. Stein; D. M. Dorsa; S. C. Woods; D. Porte

doi:10.1016/0196-9781(86)90062-8

Brain insulin binding is decreased in Wistar Kyoto rats carrying the 'fa' gene

D. P. Figlewicz, H. Ikeda, T. R. Hunt, L. J. Stein, D. M. Dorsa, S. C. Woods, D. Porte

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

We have previously reported that insulin binding is decreased in the olfactory bulb of both heterozygous (Fa/fa) and obese (fa/fa) Zucker rats. In the present study, we measured insulin binding in membranes prepared from the olfactory bulb, cerebral cortex, and hypothalamus of control (Fa/Fa) Wistar Kyoto rats; "fatty" (fa/fa) Wistar Kyoto rats; and phenotypically lean (Fa/?) Wistar Kyoto rats. Insulin binding was decreased in all brain regions, as well as the liver of the obese Wistar Kyoto fa/fa rats. Additionally, insulin binding was decreased in the liver and brain membranes from the Fa/? Wistar Kyoto rats. As most of the Fa/? rats were probably carriers of one 'fa' gene, but the population was only slightly hyperinsulinemic, we conclude that-as in the Zucker rat-it is the presence and expression of the 'fa' gene rather than downregulation which results in the decreased insulin binding. Thus, regulation of the brain insulin receptor appears to be independent of plasma or cerebrospinal fluid insulin levels.

Original language	English (US)
Pages (from-to)	61-65
Number of pages	5
Journal	Peptides
Volume	7
Issue number	1
DOIs	https://doi.org/10.1016/0196-9781(86)90062-8
State	Published - 1986
Externally published	Yes

Keywords

Body weight regulation
Brain insulin receptors
Obesity
Wistar 'fatty' rat

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

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10.1016/0196-9781(86)90062-8

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title = "Brain insulin binding is decreased in Wistar Kyoto rats carrying the 'fa' gene",

abstract = "We have previously reported that insulin binding is decreased in the olfactory bulb of both heterozygous (Fa/fa) and obese (fa/fa) Zucker rats. In the present study, we measured insulin binding in membranes prepared from the olfactory bulb, cerebral cortex, and hypothalamus of control (Fa/Fa) Wistar Kyoto rats; {"}fatty{"} (fa/fa) Wistar Kyoto rats; and phenotypically lean (Fa/?) Wistar Kyoto rats. Insulin binding was decreased in all brain regions, as well as the liver of the obese Wistar Kyoto fa/fa rats. Additionally, insulin binding was decreased in the liver and brain membranes from the Fa/? Wistar Kyoto rats. As most of the Fa/? rats were probably carriers of one 'fa' gene, but the population was only slightly hyperinsulinemic, we conclude that-as in the Zucker rat-it is the presence and expression of the 'fa' gene rather than downregulation which results in the decreased insulin binding. Thus, regulation of the brain insulin receptor appears to be independent of plasma or cerebrospinal fluid insulin levels.",

keywords = "Body weight regulation, Brain insulin receptors, Obesity, Wistar 'fatty' rat",

author = "Figlewicz, {D. P.} and H. Ikeda and Hunt, {T. R.} and Stein, {L. J.} and Dorsa, {D. M.} and Woods, {S. C.} and D. Porte",

note = "Funding Information: The authors gratefully acknowledge Sharon Oliver-Clifner and Lylian Merkley for excellent technical assistance, and Sheila Day and Lynn Guthrie for expert assistance in manuscript preparation. Support for these studies was provided by Public Health Service Grants AM 17047, AM 12829, AM 17844, a Research Career Development Award from the American Diabetes Association (Dr. Fig-lewicz), and the Veterans Administration. The findings were presented in preliminary form at the annual meeting of the Society for Neurosciences, Anaheim, CA 1984.",

year = "1986",

doi = "10.1016/0196-9781(86)90062-8",

language = "English (US)",

volume = "7",

pages = "61--65",

journal = "Peptides",

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T1 - Brain insulin binding is decreased in Wistar Kyoto rats carrying the 'fa' gene

AU - Figlewicz, D. P.

AU - Ikeda, H.

AU - Hunt, T. R.

AU - Stein, L. J.

AU - Dorsa, D. M.

AU - Woods, S. C.

AU - Porte, D.

N1 - Funding Information: The authors gratefully acknowledge Sharon Oliver-Clifner and Lylian Merkley for excellent technical assistance, and Sheila Day and Lynn Guthrie for expert assistance in manuscript preparation. Support for these studies was provided by Public Health Service Grants AM 17047, AM 12829, AM 17844, a Research Career Development Award from the American Diabetes Association (Dr. Fig-lewicz), and the Veterans Administration. The findings were presented in preliminary form at the annual meeting of the Society for Neurosciences, Anaheim, CA 1984.

PY - 1986

Y1 - 1986

N2 - We have previously reported that insulin binding is decreased in the olfactory bulb of both heterozygous (Fa/fa) and obese (fa/fa) Zucker rats. In the present study, we measured insulin binding in membranes prepared from the olfactory bulb, cerebral cortex, and hypothalamus of control (Fa/Fa) Wistar Kyoto rats; "fatty" (fa/fa) Wistar Kyoto rats; and phenotypically lean (Fa/?) Wistar Kyoto rats. Insulin binding was decreased in all brain regions, as well as the liver of the obese Wistar Kyoto fa/fa rats. Additionally, insulin binding was decreased in the liver and brain membranes from the Fa/? Wistar Kyoto rats. As most of the Fa/? rats were probably carriers of one 'fa' gene, but the population was only slightly hyperinsulinemic, we conclude that-as in the Zucker rat-it is the presence and expression of the 'fa' gene rather than downregulation which results in the decreased insulin binding. Thus, regulation of the brain insulin receptor appears to be independent of plasma or cerebrospinal fluid insulin levels.

AB - We have previously reported that insulin binding is decreased in the olfactory bulb of both heterozygous (Fa/fa) and obese (fa/fa) Zucker rats. In the present study, we measured insulin binding in membranes prepared from the olfactory bulb, cerebral cortex, and hypothalamus of control (Fa/Fa) Wistar Kyoto rats; "fatty" (fa/fa) Wistar Kyoto rats; and phenotypically lean (Fa/?) Wistar Kyoto rats. Insulin binding was decreased in all brain regions, as well as the liver of the obese Wistar Kyoto fa/fa rats. Additionally, insulin binding was decreased in the liver and brain membranes from the Fa/? Wistar Kyoto rats. As most of the Fa/? rats were probably carriers of one 'fa' gene, but the population was only slightly hyperinsulinemic, we conclude that-as in the Zucker rat-it is the presence and expression of the 'fa' gene rather than downregulation which results in the decreased insulin binding. Thus, regulation of the brain insulin receptor appears to be independent of plasma or cerebrospinal fluid insulin levels.

KW - Body weight regulation

KW - Brain insulin receptors

KW - Obesity

KW - Wistar 'fatty' rat

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Brain insulin binding is decreased in Wistar Kyoto rats carrying the 'fa' gene

Abstract

Keywords

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