TY - JOUR
T1 - C2H2 zinc finger-SET histone methyltransferase is a plant-specific chromatin modifier
AU - Krichevsky, Alexander
AU - Gutgarts, Helen
AU - Kozlovsky, Stanislav V.
AU - Tzfira, Tzvi
AU - Sutton, Ann
AU - Sternglanz, Rolf
AU - Mandel, Gail
AU - Citovsky, Vitaly
PY - 2007/3/1
Y1 - 2007/3/1
N2 - Histone modification represents a universal mechanism for regulation of eukaryotic gene expression underlying diverse biological processes from neuronal gene expression in mammals to control of flowering in plants. In animal cells, these chromatin modifications are effected by well-defined multiprotein complexes containing specific histone-modifying activities. In plants, information about the composition of such co-repressor complexes is just beginning to emerge. Here, we report that two Arabidopsis thaliana factors, a SWIRM domain polyamine oxidase protein, AtSWP1, and a plant-specific C2H2 zinc finger-SET domain protein, AtCZS, interact with each other in plant cells and repress expression of a negative regulator of flowering, FLOWERING LOCUS C (FLC) via an autonomous, vernalization-independent pathway. Loss-of-function of either AtSWP1 or AtCZS results in reduced dimethylation of lysine 9 and lysine 27 of histone H3 and hyperacetylation of histone H4 within the FLC locus, in elevated FLC mRNA levels, and in moderately delayed flowering. Thus, AtSWP1 and AtCZS represent two main components of a co-repressor complex that fine tunes flowering and is unique to plants.
AB - Histone modification represents a universal mechanism for regulation of eukaryotic gene expression underlying diverse biological processes from neuronal gene expression in mammals to control of flowering in plants. In animal cells, these chromatin modifications are effected by well-defined multiprotein complexes containing specific histone-modifying activities. In plants, information about the composition of such co-repressor complexes is just beginning to emerge. Here, we report that two Arabidopsis thaliana factors, a SWIRM domain polyamine oxidase protein, AtSWP1, and a plant-specific C2H2 zinc finger-SET domain protein, AtCZS, interact with each other in plant cells and repress expression of a negative regulator of flowering, FLOWERING LOCUS C (FLC) via an autonomous, vernalization-independent pathway. Loss-of-function of either AtSWP1 or AtCZS results in reduced dimethylation of lysine 9 and lysine 27 of histone H3 and hyperacetylation of histone H4 within the FLC locus, in elevated FLC mRNA levels, and in moderately delayed flowering. Thus, AtSWP1 and AtCZS represent two main components of a co-repressor complex that fine tunes flowering and is unique to plants.
KW - Chromatin remodeling
KW - Gene repression
KW - Histone modification
UR - http://www.scopus.com/inward/record.url?scp=33846907165&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846907165&partnerID=8YFLogxK
U2 - 10.1016/j.ydbio.2006.11.012
DO - 10.1016/j.ydbio.2006.11.012
M3 - Article
C2 - 17224141
AN - SCOPUS:33846907165
SN - 0012-1606
VL - 303
SP - 259
EP - 269
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -