@article{2419f6a61fe449a987e550ff41258261,
title = "Capsid-specific nanobody effects on HIV-1 assembly and infectivity",
abstract = "The capsid (CA) domain of the HIV-1 precursor Gag (PrGag) protein plays multiple roles in HIV-1 replication, and is central to the assembly of immature virions, and mature virus cores. CA proteins themselves are composed of N-terminal domains (NTDs) and C-terminal domains (CTDs). We have investigated the interactions of CA with anti-CA nanobodies, which derive from the antigen recognition regions of camelid heavy chain-only antibodies. The one CA NTD-specific and two CTD-specific nanobodies we analyzed proved sensitive and specific HIV-1 CA detection reagents in immunoassays. When co-expressed with HIV-1 Gag proteins in cells, the NTD-specific nanobody was efficiently assembled into virions and did not perturb virus assembly. In contrast, the two CTD-specific nanobodies reduced PrGag processing, virus release and HIV-1 infectivity. Our results demonstrate the feasibility of Gag-targeted nanobody inhibition of HIV-1.",
keywords = "Capsid, Gag, HIV-1, Nanobody",
author = "Ayna Alfadhli and Romanaggi, {Ce Ann} and Barklis, {Robin Lid} and Ilaria Merutka and Bates, {Timothy A.} and Tafesse, {Fikadu G.} and Eric Barklis",
note = "Funding Information: E. B. and A. A. gratefully acknowledge support from the Medical Research Foundation of Oregon and from the National Institutes of Health (NIH; R01 AI152579). F. T. was supported by NIH grants R01 AI141549 and T32 AI747225. We thank Drs. David Kabat and Bruce Cheesebro respectively for supplying the HiJ and hybridoma (Hy183, Hy187) cell lines. We also thank all members of Barklis and Tafesse labs, and to the Vollum Institute support staff for their help and assistance. Funding Information: E. B. and A. A. gratefully acknowledge support from the Medical Research Foundation of Oregon and from the National Institutes of Health (NIH; R01 AI152579). F. T. was supported by NIH grants R01 AI141549 and T32 AI747225. We thank Drs. David Kabat and Bruce Cheesebro respectively for supplying the HiJ and hybridoma (Hy183, Hy187) cell lines. We also thank all members of Barklis and Tafesse labs, and to the Vollum Institute support staff for their help and assistance. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = oct,
doi = "10.1016/j.virol.2021.07.001",
language = "English (US)",
volume = "562",
pages = "19--28",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
}