Cargo of kinesin identified as JIP scaffolding proteins and associated signaling molecules

Kristen J. Verhey, Debra Meyer, Reneé Deehan, John Blenis, Bruce J. Schnapp, Tom A. Rapoport, Ben Margolis

Research output: Contribution to journalArticlepeer-review

501 Scopus citations


The cargo that the molecular motor kinesin moves along microtubules has been elusive. We searched for binding partners of the COOH terminus of kinesin light chain, which contains tetratricopeptide repeat (TPR) motifs. Three proteins were found, the c-jun NH2-terminal kinase (JNK)-interacting proteins (JIPs) JIP-1, JIP-2, and JIP-3, which are scaffolding proteins for the JNK signaling pathway. Concentration of JIPs in nerve terminals requires kinesin, as evident from the analysis of JIP COOH-terminal mutants and dominant negative kinesin constructs. Coprecipitation experiments suggest that kinesin carries the JIP scaffolds preloaded with cytoplasmic (dual leucine zipper-bearing kinase) and transmembrane signaling molecules (the Reelin receptor, ApoER2). These results demonstrate a direct interaction between conventional kinesin and a cargo, indicate that motor proteins are linked to their membranous cargo via scaffolding proteins, and support a role for motor proteins in spatial regulation of signal transduction pathways.

Original languageEnglish (US)
Pages (from-to)959-970
Number of pages12
JournalJournal of Cell Biology
Issue number5
StatePublished - Mar 5 2001
Externally publishedYes


  • JNK signaling
  • Kinesin
  • Molecular motors
  • Reelin
  • Scaffolding protein

ASJC Scopus subject areas

  • Cell Biology


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