TY - JOUR
T1 - Cell-cycle characteristics of undifferentiated and differentiating embryonal carcinoma cells
AU - Rosenstraus, Maurice J.
AU - Sundell, Cynthia L.
AU - Liskay, R. Michael
N1 - Funding Information:
AntiSSEAl serum and anti-laminin serum were generously provided by Dr. D. Solter and Dr. R. Oshima, respectively. The expert technical assistance of Laura Kupatt and Nelda Davis was greatly appreciated.W e thank Dr. David Axelrod and Dr. Helena Axelrod for their valuable suggestionsr egarding the manuscript. This work was supported by Research Grant CA-26014f rom National Cancer Institute (to M.J.R) and NIH Research Grant GM 25870( to R.M.L.).
PY - 1982/2
Y1 - 1982/2
N2 - The cell-cycle parameters of undifferentiated and differentiating embryonal carcinoma cells were determined. Undifferentiated cultures of an F9 subclone, OTF9-63, had a 12-hr generation time, 10-hr S, and 2-hr G2 + M. G1 was less than 0.5 hr. In contrast, OTF9-63 cells induced to differentiate by treatment with retinoic acid had a 16.8-hr generation time, 12.5-hr S, 2-hr G2 + M, and 2.3-hr G1. Similar results were obtained with undifferentiated cultures and aggregation-induced differentiating cultures of PSA-1 cells. These data demonstrate that the undifferentiated stem cells have little or no G1, and that both G1 and S lengthen during differentiation.
AB - The cell-cycle parameters of undifferentiated and differentiating embryonal carcinoma cells were determined. Undifferentiated cultures of an F9 subclone, OTF9-63, had a 12-hr generation time, 10-hr S, and 2-hr G2 + M. G1 was less than 0.5 hr. In contrast, OTF9-63 cells induced to differentiate by treatment with retinoic acid had a 16.8-hr generation time, 12.5-hr S, 2-hr G2 + M, and 2.3-hr G1. Similar results were obtained with undifferentiated cultures and aggregation-induced differentiating cultures of PSA-1 cells. These data demonstrate that the undifferentiated stem cells have little or no G1, and that both G1 and S lengthen during differentiation.
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U2 - 10.1016/0012-1606(82)90340-2
DO - 10.1016/0012-1606(82)90340-2
M3 - Article
C2 - 7056445
AN - SCOPUS:0020094203
SN - 0012-1606
VL - 89
SP - 516
EP - 520
JO - Developmental Biology
JF - Developmental Biology
IS - 2
ER -