TY - JOUR
T1 - Cell cycle features of primate embryonic stem cells
AU - Fluckiger, Anne Catherine
AU - Marcy, Guillaume
AU - Marchand, Mélanie
AU - Négre, Didier
AU - Cosset, François Loïc
AU - Mitalipov, Shoukhrat
AU - Wolf, Don
AU - Savatier, Pierre
AU - Dehay, Colette
PY - 2006/3
Y1 - 2006/3
N2 - Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit through the G1 phase, which accounts for 15% of the total cell cycle duration. Monkey ESCs exhibit a non-phasic expression of cyclin E, which is detected during all phases of the cell cycle, and do not growth-arrest in G1 after γ-irradiation, reflecting the absence of a G1 checkpoint. Serum deprivation or pharmacological inhibition of mitogen-activated protein kinase kinase (MEK) did not result in any alteration in the cell cycle distribution, indicating that ESC growth does not rely on mitogenic signals transduced by the Ras/Raf/MEK pathway. Taken together, these data indicate that rhesus monkey ESCs, like their murine counterparts, exhibit unusual cell cycle features in which cell cycle control mechanisms operating during the G1 phase are reduced or absent.
AB - Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit through the G1 phase, which accounts for 15% of the total cell cycle duration. Monkey ESCs exhibit a non-phasic expression of cyclin E, which is detected during all phases of the cell cycle, and do not growth-arrest in G1 after γ-irradiation, reflecting the absence of a G1 checkpoint. Serum deprivation or pharmacological inhibition of mitogen-activated protein kinase kinase (MEK) did not result in any alteration in the cell cycle distribution, indicating that ESC growth does not rely on mitogenic signals transduced by the Ras/Raf/MEK pathway. Taken together, these data indicate that rhesus monkey ESCs, like their murine counterparts, exhibit unusual cell cycle features in which cell cycle control mechanisms operating during the G1 phase are reduced or absent.
KW - Cell cycle
KW - Rhesus embryonic stem cells
KW - Stemness
UR - http://www.scopus.com/inward/record.url?scp=33744975288&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33744975288&partnerID=8YFLogxK
U2 - 10.1634/stemcells.2005-0194
DO - 10.1634/stemcells.2005-0194
M3 - Article
C2 - 16239321
AN - SCOPUS:33744975288
SN - 1066-5099
VL - 24
SP - 547
EP - 556
JO - Stem Cells
JF - Stem Cells
IS - 3
ER -