TY - JOUR
T1 - Cell-free protein synthesis
T2 - The state of the art
AU - Whittaker, James W.
N1 - Funding Information:
Acknowledgments The author would like to thank Corinna Tuckey (New England Biolabs) for helpful discussions. This work was supported by the National Institutes of Health (GM42680 to J. W. W.).
PY - 2013/2
Y1 - 2013/2
N2 - Cell-free protein synthesis harnesses the synthetic power of biology, programming the ribosomal translational machinery of the cell to create macromolecular products. Like PCR, which uses cellular replication machinery to create a DNA amplifier, cell-free protein synthesis is emerging as a transformative technology with broad applications in protein engineering, biopharmaceutical development, and post-genomic research. By breaking free from the constraints of cell-based systems, it takes the next step towards synthetic biology. Recent advances in reconstituted cell-free protein synthesis (Protein synthesis Using Recombinant Elements expression systems) are creating new opportunities to tailor the reactions for specialized applications including in vitro protein evolution, printing protein microarrays, isotopic labeling, and incorporating nonnatural amino acids.
AB - Cell-free protein synthesis harnesses the synthetic power of biology, programming the ribosomal translational machinery of the cell to create macromolecular products. Like PCR, which uses cellular replication machinery to create a DNA amplifier, cell-free protein synthesis is emerging as a transformative technology with broad applications in protein engineering, biopharmaceutical development, and post-genomic research. By breaking free from the constraints of cell-based systems, it takes the next step towards synthetic biology. Recent advances in reconstituted cell-free protein synthesis (Protein synthesis Using Recombinant Elements expression systems) are creating new opportunities to tailor the reactions for specialized applications including in vitro protein evolution, printing protein microarrays, isotopic labeling, and incorporating nonnatural amino acids.
KW - Cell-free translation
KW - PURE expression
KW - Protein engineering
KW - Protein synthesis
KW - Synthetic biology
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U2 - 10.1007/s10529-012-1075-4
DO - 10.1007/s10529-012-1075-4
M3 - Review article
C2 - 23086573
AN - SCOPUS:84872807174
SN - 0141-5492
VL - 35
SP - 143
EP - 152
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 2
ER -