@article{b776347aa999443a9b9360ab77309b7b,
title = "Cell-selective metabolic labeling of proteins",
abstract = "Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.",
author = "Ngo, {John T.} and Champion, {Julie A.} and Alborz Mahdavi and Tanrikulu, {I. Caglar} and Beatty, {Kimberly E.} and Connor, {Rebecca E.} and Yoo, {Tae Hyeon} and Dieterich, {Daniela C.} and Schuman, {Erin M.} and Tirrell, {David A.}",
note = "Funding Information: We gratefully acknowledge the US National Institutes of Health (R21 DA020589 and R01 GM62523) and the US Army Research Office Institute for Collaborative Biotechnologies for support of this work. We thank K. Boulware for assistance in generating the three-dimensional movie of infected macrophages. E.M.S. is an investigator of the Howard Hughes Medical Institute.",
year = "2009",
month = oct,
doi = "10.1038/nchembio.200",
language = "English (US)",
volume = "5",
pages = "715--717",
journal = "Nature Chemical Biology",
issn = "1552-4450",
publisher = "Nature Publishing Group",
number = "10",
}