Cell-selective metabolic labeling of proteins

John T. Ngo, Julie A. Champion, Alborz Mahdavi, I. Caglar Tanrikulu, Kimberly E. Beatty, Rebecca E. Connor, Tae Hyeon Yoo, Daniela C. Dieterich, Erin M. Schuman, David A. Tirrell

Research output: Contribution to journalArticlepeer-review

139 Scopus citations


Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.

Original languageEnglish (US)
Pages (from-to)715-717
Number of pages3
JournalNature Chemical Biology
Issue number10
StatePublished - Oct 2009
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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