Cellular analysis based on moving infrared optical gradient fields

Philippe J. Marchand; Mark Wang; Catherine Schnabel; Mirianas Chachisvillis; Haichuan Zhang; Rong Yang; Laura McMullin; Norbert Hagen; Osman Kibar; Sadik Esener; Gene Tu; Sudipto Sur; Luis Pestana; Kirk Haden; Khai Truong; Wilson Seto; Kris Lykstad; Laura Simons; Michael Paliotti; Hona Kariv; Jeffrey Hall; William Butler

doi:10.1117/12.509376

Cellular analysis based on moving infrared optical gradient fields

Philippe J. Marchand, Mark Wang, Catherine Schnabel, Mirianas Chachisvillis, Haichuan Zhang, Rong Yang, Laura McMullin, Norbert Hagen, Osman Kibar, Sadik Esener, Gene Tu, Sudipto Sur, Luis Pestana, Kirk Haden, Khai Truong, Wilson Seto, Kris Lykstad, Laura Simons, Michael Paliotti, Hona KarivJeffrey Hall, William Butler

Research output: Contribution to journal › Conference article › peer-review

Abstract

A novel, non-invasive measurement technique for quantitative cellular analysis is presented that utilizes the forces generated by an optical beam to evaluate the physical properties of live cells in suspension. Analysis is performed by rapidly scanning a focused, near-infrared laser line with a high cross-sectional intensity gradient across a field of cells and monitoring their interaction with the beam. The response of each cell to the laser depends on its size, structure, morphology, composition, and surface membrane properties; therefore, using this technique, cell populations of different type, treatment, or biological state can be compared. To demonstrate the utility of this cell analysis platform we have evaluated the early stages of apoptosis induced in the U937 cancer cell line by the drug camptothecin and compared the results to established references assays. Measurements on our platform show detection of cellular changes earlier than either of the fluorescence-based annexin V or caspase assays. Because no labeling or additional cell processing is required and because accurate assays can be performed with a small number of cells, this measurement technique may find suitable applications in cell research, medical diagnostics, and drug discovery.

Original language	English (US)
Pages (from-to)	42-50
Number of pages	9
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5181
DOIs	https://doi.org/10.1117/12.509376
State	Published - 2003
Externally published	Yes
Event	Wave Optics and Photonic Devices for Optical Information Processing II - San Diego, CA, United States Duration: Aug 7 2003 → Aug 8 2003

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.509376

Cite this

Marchand, P. J., Wang, M., Schnabel, C., Chachisvillis, M., Zhang, H., Yang, R., McMullin, L., Hagen, N., Kibar, O., Esener, S., Tu, G., Sur, S., Pestana, L., Haden, K., Truong, K., Seto, W., Lykstad, K., Simons, L., Paliotti, M., ... Butler, W. (2003). Cellular analysis based on moving infrared optical gradient fields. Proceedings of SPIE - The International Society for Optical Engineering, 5181, 42-50. https://doi.org/10.1117/12.509376

Marchand, PJ, Wang, M, Schnabel, C, Chachisvillis, M, Zhang, H, Yang, R, McMullin, L, Hagen, N, Kibar, O, Esener, S, Tu, G, Sur, S, Pestana, L, Haden, K, Truong, K, Seto, W, Lykstad, K, Simons, L, Paliotti, M, Kariv, H, Hall, J & Butler, W 2003, 'Cellular analysis based on moving infrared optical gradient fields', Proceedings of SPIE - The International Society for Optical Engineering, vol. 5181, pp. 42-50. https://doi.org/10.1117/12.509376

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title = "Cellular analysis based on moving infrared optical gradient fields",

abstract = "A novel, non-invasive measurement technique for quantitative cellular analysis is presented that utilizes the forces generated by an optical beam to evaluate the physical properties of live cells in suspension. Analysis is performed by rapidly scanning a focused, near-infrared laser line with a high cross-sectional intensity gradient across a field of cells and monitoring their interaction with the beam. The response of each cell to the laser depends on its size, structure, morphology, composition, and surface membrane properties; therefore, using this technique, cell populations of different type, treatment, or biological state can be compared. To demonstrate the utility of this cell analysis platform we have evaluated the early stages of apoptosis induced in the U937 cancer cell line by the drug camptothecin and compared the results to established references assays. Measurements on our platform show detection of cellular changes earlier than either of the fluorescence-based annexin V or caspase assays. Because no labeling or additional cell processing is required and because accurate assays can be performed with a small number of cells, this measurement technique may find suitable applications in cell research, medical diagnostics, and drug discovery.",

author = "Marchand, {Philippe J.} and Mark Wang and Catherine Schnabel and Mirianas Chachisvillis and Haichuan Zhang and Rong Yang and Laura McMullin and Norbert Hagen and Osman Kibar and Sadik Esener and Gene Tu and Sudipto Sur and Luis Pestana and Kirk Haden and Khai Truong and Wilson Seto and Kris Lykstad and Laura Simons and Michael Paliotti and Hona Kariv and Jeffrey Hall and William Butler",

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language = "English (US)",

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T1 - Cellular analysis based on moving infrared optical gradient fields

AU - Marchand, Philippe J.

AU - Wang, Mark

AU - Schnabel, Catherine

AU - Chachisvillis, Mirianas

AU - Zhang, Haichuan

AU - Yang, Rong

AU - McMullin, Laura

AU - Hagen, Norbert

AU - Kibar, Osman

AU - Esener, Sadik

AU - Tu, Gene

AU - Sur, Sudipto

AU - Pestana, Luis

AU - Haden, Kirk

AU - Truong, Khai

AU - Seto, Wilson

AU - Lykstad, Kris

AU - Simons, Laura

AU - Paliotti, Michael

AU - Kariv, Hona

AU - Hall, Jeffrey

AU - Butler, William

PY - 2003

Y1 - 2003

N2 - A novel, non-invasive measurement technique for quantitative cellular analysis is presented that utilizes the forces generated by an optical beam to evaluate the physical properties of live cells in suspension. Analysis is performed by rapidly scanning a focused, near-infrared laser line with a high cross-sectional intensity gradient across a field of cells and monitoring their interaction with the beam. The response of each cell to the laser depends on its size, structure, morphology, composition, and surface membrane properties; therefore, using this technique, cell populations of different type, treatment, or biological state can be compared. To demonstrate the utility of this cell analysis platform we have evaluated the early stages of apoptosis induced in the U937 cancer cell line by the drug camptothecin and compared the results to established references assays. Measurements on our platform show detection of cellular changes earlier than either of the fluorescence-based annexin V or caspase assays. Because no labeling or additional cell processing is required and because accurate assays can be performed with a small number of cells, this measurement technique may find suitable applications in cell research, medical diagnostics, and drug discovery.

AB - A novel, non-invasive measurement technique for quantitative cellular analysis is presented that utilizes the forces generated by an optical beam to evaluate the physical properties of live cells in suspension. Analysis is performed by rapidly scanning a focused, near-infrared laser line with a high cross-sectional intensity gradient across a field of cells and monitoring their interaction with the beam. The response of each cell to the laser depends on its size, structure, morphology, composition, and surface membrane properties; therefore, using this technique, cell populations of different type, treatment, or biological state can be compared. To demonstrate the utility of this cell analysis platform we have evaluated the early stages of apoptosis induced in the U937 cancer cell line by the drug camptothecin and compared the results to established references assays. Measurements on our platform show detection of cellular changes earlier than either of the fluorescence-based annexin V or caspase assays. Because no labeling or additional cell processing is required and because accurate assays can be performed with a small number of cells, this measurement technique may find suitable applications in cell research, medical diagnostics, and drug discovery.

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ER -

Cellular analysis based on moving infrared optical gradient fields

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