Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n

Bernhard Weber; Colin Collins; Carolyn Robbins; R. Ellen Magenis; Allen D. Delaney; Joe W. Gray; Michael R. Hayden

doi:10.1093/nar/18.11.3353

Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)_n

Bernhard Weber, Colin Collins, Carolyn Robbins, R. Ellen Magenis, Allen D. Delaney, Joe W. Gray, Michael R. Hayden

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

We present a strategy for the cloning of DNA sequences adjacent to the tandemly repeated DNA sequence (TTAGGG)_n. Sequence analysis of 14 independently isolated clones revealed the presence of non-repetitive sequences immediately adjacent to or flanked by blocks of the simple repeat (TTAGGG)_n. In addition, we provide sequence information on two previously undescribed tandemly repeated sequences, including a 9 bp repeat and a modification of the (TTAGGG)_n repeat. Using different mapping approaches six sub-clones, free of the TTAGGG repeat, were assigned to a single human chromosome. Moreover, in situ hybridization mapped one of these subclones, G2 - 1H, definitively to the telomeric band on chromosome 4q. However, Bal 31 insensitivity suggests a location in a more subterminal region. All the (TTAGGG)_n-adjacent unique sequences tested are highly conserved among primates but are not present in other mammalian species. Identification and mapping of TTAGGG-adjacent sequences will provide a refined insight into the genomic organization of the (TTAGGG)_nrepeat. The isolation of chromosome specific TTAGGG-adjacent sequences from subtelomeric regions of all human chromosomes will serve as important end points for the genetic maps and will be useful for the molecular characterization of chromosomal rearrangements involving telomeres.

Original language	English (US)
Pages (from-to)	3353-3361
Number of pages	9
Journal	Nucleic acids research
Volume	18
Issue number	11
DOIs	https://doi.org/10.1093/nar/18.11.3353
State	Published - Jun 11 1990
Externally published	Yes

ASJC Scopus subject areas

Genetics

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@article{25990f693ae64a83a12e17ba878efc43,

title = "Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n",

abstract = "We present a strategy for the cloning of DNA sequences adjacent to the tandemly repeated DNA sequence (TTAGGG)n. Sequence analysis of 14 independently isolated clones revealed the presence of non-repetitive sequences immediately adjacent to or flanked by blocks of the simple repeat (TTAGGG)n. In addition, we provide sequence information on two previously undescribed tandemly repeated sequences, including a 9 bp repeat and a modification of the (TTAGGG)n repeat. Using different mapping approaches six sub-clones, free of the TTAGGG repeat, were assigned to a single human chromosome. Moreover, in situ hybridization mapped one of these subclones, G2 - 1H, definitively to the telomeric band on chromosome 4q. However, Bal 31 insensitivity suggests a location in a more subterminal region. All the (TTAGGG)n-adjacent unique sequences tested are highly conserved among primates but are not present in other mammalian species. Identification and mapping of TTAGGG-adjacent sequences will provide a refined insight into the genomic organization of the (TTAGGG)nrepeat. The isolation of chromosome specific TTAGGG-adjacent sequences from subtelomeric regions of all human chromosomes will serve as important end points for the genetic maps and will be useful for the molecular characterization of chromosomal rearrangements involving telomeres.",

author = "Bernhard Weber and Colin Collins and Carolyn Robbins and Magenis, {R. Ellen} and Delaney, {Allen D.} and Gray, {Joe W.} and Hayden, {Michael R.}",

note = "Funding Information: The authors wish to thank Drs G.Bruns, Boston and P.Goodfellow, Vancouver for kindly providing somatic cell hybrid panels and Dr P.Goodfellow for critical comments on the manuscript. We thank S.Howe and K.Jack for secretarial assistance and C.Munro for preparation of the figures. B.W. is supported by a fellowship from the Deutsche Forschungs-gemeinschaft (We 1259/1-1). C.C. is a predoctoral student of the Huntington Society of Canada. This work has been supported by MRC (Canada No. 5-99133).",

year = "1990",

month = jun,

day = "11",

doi = "10.1093/nar/18.11.3353",

language = "English (US)",

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pages = "3353--3361",

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TY - JOUR

T1 - Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)n

AU - Weber, Bernhard

AU - Collins, Colin

AU - Robbins, Carolyn

AU - Magenis, R. Ellen

AU - Delaney, Allen D.

AU - Gray, Joe W.

AU - Hayden, Michael R.

N1 - Funding Information: The authors wish to thank Drs G.Bruns, Boston and P.Goodfellow, Vancouver for kindly providing somatic cell hybrid panels and Dr P.Goodfellow for critical comments on the manuscript. We thank S.Howe and K.Jack for secretarial assistance and C.Munro for preparation of the figures. B.W. is supported by a fellowship from the Deutsche Forschungs-gemeinschaft (We 1259/1-1). C.C. is a predoctoral student of the Huntington Society of Canada. This work has been supported by MRC (Canada No. 5-99133).

PY - 1990/6/11

Y1 - 1990/6/11

N2 - We present a strategy for the cloning of DNA sequences adjacent to the tandemly repeated DNA sequence (TTAGGG)n. Sequence analysis of 14 independently isolated clones revealed the presence of non-repetitive sequences immediately adjacent to or flanked by blocks of the simple repeat (TTAGGG)n. In addition, we provide sequence information on two previously undescribed tandemly repeated sequences, including a 9 bp repeat and a modification of the (TTAGGG)n repeat. Using different mapping approaches six sub-clones, free of the TTAGGG repeat, were assigned to a single human chromosome. Moreover, in situ hybridization mapped one of these subclones, G2 - 1H, definitively to the telomeric band on chromosome 4q. However, Bal 31 insensitivity suggests a location in a more subterminal region. All the (TTAGGG)n-adjacent unique sequences tested are highly conserved among primates but are not present in other mammalian species. Identification and mapping of TTAGGG-adjacent sequences will provide a refined insight into the genomic organization of the (TTAGGG)nrepeat. The isolation of chromosome specific TTAGGG-adjacent sequences from subtelomeric regions of all human chromosomes will serve as important end points for the genetic maps and will be useful for the molecular characterization of chromosomal rearrangements involving telomeres.

AB - We present a strategy for the cloning of DNA sequences adjacent to the tandemly repeated DNA sequence (TTAGGG)n. Sequence analysis of 14 independently isolated clones revealed the presence of non-repetitive sequences immediately adjacent to or flanked by blocks of the simple repeat (TTAGGG)n. In addition, we provide sequence information on two previously undescribed tandemly repeated sequences, including a 9 bp repeat and a modification of the (TTAGGG)n repeat. Using different mapping approaches six sub-clones, free of the TTAGGG repeat, were assigned to a single human chromosome. Moreover, in situ hybridization mapped one of these subclones, G2 - 1H, definitively to the telomeric band on chromosome 4q. However, Bal 31 insensitivity suggests a location in a more subterminal region. All the (TTAGGG)n-adjacent unique sequences tested are highly conserved among primates but are not present in other mammalian species. Identification and mapping of TTAGGG-adjacent sequences will provide a refined insight into the genomic organization of the (TTAGGG)nrepeat. The isolation of chromosome specific TTAGGG-adjacent sequences from subtelomeric regions of all human chromosomes will serve as important end points for the genetic maps and will be useful for the molecular characterization of chromosomal rearrangements involving telomeres.

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U2 - 10.1093/nar/18.11.3353

DO - 10.1093/nar/18.11.3353

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JO - Nucleic acids research

JF - Nucleic acids research

IS - 11

ER -

Characterization and organization of DNA sequences adjacent to the human telomere associated repeat (TTAGGG)_n

Abstract

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