Characterization of a mutant mouse lymphoma cell with deficient transport of purine and pyrimidine nucleosides

A. Cohen, B. Ullman, D. W. Martin

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

A clone (AE1) of cells resistant to adenosine cytotoxicity was isolated from a mutagenized population of mouse T-lymphoma (S49) cells. These adenosine-resistant cells cannot transport adenosine or any other purine or pyrimidine nucleoside tested, but their transport of purine bases is intact. The AE1 cells also have an impaired ability to salvage thymidine. Kinetic analyses of the uptake of purine and pyrimidine nucleosides further substantiate the evidence that the facilitated transport of purine and pyrimidine nucleosides in S49 cells is mediated by the same carrier. Growth rate and transport studies indicate that the lack of expression of the nucleoside transport in AE1 cells is recessive in intraspecies cell-cell hybrids.

Original languageEnglish (US)
Pages (from-to)112-116
Number of pages5
JournalJournal of Biological Chemistry
Volume254
Issue number1
StatePublished - 1979
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Characterization of a mutant mouse lymphoma cell with deficient transport of purine and pyrimidine nucleosides'. Together they form a unique fingerprint.

Cite this