TY - JOUR
T1 - Characterization of human platelet binding of recombinant T cell receptor ligand
AU - Itakura, Asako
AU - Aslan, Joseph E.
AU - Sinha, Sushmita
AU - White-Adams, Tara C.
AU - Patel, Ishan A.
AU - Meza-Romero, Roberto
AU - Vandenbark, Arthur A.
AU - Burrows, Gregory G.
AU - Offner, Halina
AU - McCarty, Owen J.T.
N1 - Funding Information:
The authors wish to thank J. Pang for experimental assistance. Dr. Sinha is a Postdoctoral Fellow of the National Multiple Sclerosis Society. This work was supported in part by the National Multiple Sclerosis Society (FG1749-A-1, RG3794-A-4 and RG3794-B-6), the National Institute of Health Grants T32 HL00778118, NS47661 and AI43960, the American Heart Association (09PRE2230117, 0910025G), and the Biomedical Laboratory R&D Service, Department of Veterans Affairs. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Multiple Sclerosis Society, American Heart Association, the National Institutes of Health, or the Department of Veterans Affairs.
PY - 2010/11/8
Y1 - 2010/11/8
N2 - Background: Recombinant T cell receptor ligands (RTLs) are bio-engineered molecules that may serve as novel therapeutic agents for the treatment of neuroinflammatory conditions such as multiple sclerosis (MS). RTLs contain membrane distal α1 plus β1 domains of class II major histocompatibility complex linked covalently to specific peptides that can be used to regulate T cell responses and inhibit experimental autoimmune encephalomyelitis (EAE). The mechanisms by which RTLs impede local recruitment and retention of inflammatory cells in the CNS, however, are not completely understood.Methods: We have recently shown that RTLs bind strongly to B cells, macrophages, and dendritic cells, but not to T cells, in an antigenic-independent manner, raising the question whether peripheral blood cells express a distinct RTL-receptor. Our study was designed to characterize the molecular mechanisms by which RTLs bind human blood platelets, and the ability of RTL to modulate platelet function.Results: Our data demonstrate that human blood platelets support binding of RTL. Immobilized RTL initiated platelet intracellular calcium mobilization and lamellipodia formation through a pathway dependent upon Src and PI3 kinases signaling. The presence of RTL in solution reduced platelet aggregation by collagen, while treatment of whole blood with RTL prolonged occlusive thrombus formation on collagen.Conclusions: Platelets, well-known regulators of hemostasis and thrombosis, have been implicated in playing a major role in inflammation and immunity. This study provides the first evidence that blood platelets express a functional RTL-receptor with a putative role in modulating pathways of neuroinflammation.
AB - Background: Recombinant T cell receptor ligands (RTLs) are bio-engineered molecules that may serve as novel therapeutic agents for the treatment of neuroinflammatory conditions such as multiple sclerosis (MS). RTLs contain membrane distal α1 plus β1 domains of class II major histocompatibility complex linked covalently to specific peptides that can be used to regulate T cell responses and inhibit experimental autoimmune encephalomyelitis (EAE). The mechanisms by which RTLs impede local recruitment and retention of inflammatory cells in the CNS, however, are not completely understood.Methods: We have recently shown that RTLs bind strongly to B cells, macrophages, and dendritic cells, but not to T cells, in an antigenic-independent manner, raising the question whether peripheral blood cells express a distinct RTL-receptor. Our study was designed to characterize the molecular mechanisms by which RTLs bind human blood platelets, and the ability of RTL to modulate platelet function.Results: Our data demonstrate that human blood platelets support binding of RTL. Immobilized RTL initiated platelet intracellular calcium mobilization and lamellipodia formation through a pathway dependent upon Src and PI3 kinases signaling. The presence of RTL in solution reduced platelet aggregation by collagen, while treatment of whole blood with RTL prolonged occlusive thrombus formation on collagen.Conclusions: Platelets, well-known regulators of hemostasis and thrombosis, have been implicated in playing a major role in inflammation and immunity. This study provides the first evidence that blood platelets express a functional RTL-receptor with a putative role in modulating pathways of neuroinflammation.
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U2 - 10.1186/1742-2094-7-75
DO - 10.1186/1742-2094-7-75
M3 - Article
C2 - 21059245
AN - SCOPUS:78049459696
SN - 1742-2094
VL - 7
JO - Journal of Neuroinflammation
JF - Journal of Neuroinflammation
M1 - 75
ER -