TY - JOUR
T1 - Characterization of insulin-like growth factor-binding protein-related protein-1 in prostate cells
AU - Vivian, Hwa
AU - Tomasini-Sprenger, Cindy
AU - López Bermejo, Abel
AU - Rosenfeld, Ron G.
AU - Plymate, Steven R.
PY - 1998
Y1 - 1998
N2 - Insulin-like growth factor-binding protein-related protein-1 (IGFBP- rP1; also known as Mac25, TAF, and PSF) is a member of the IGFBP superfamily. It is a cystein-rich protein that shares structural and functional similarities with the conventional IGFBPs. In situ hybridization of prostate tissue sections Show intense IGFBP-rP1 messenger ribonucleic acid (mRNA) expression in normal stroma and glandular epithelium. There was a significant loss of detectable IGFBP-rP1 mRNA in metastatic prostate tissue. IGFBP-rP1 mRNA (Northern blots) and protein (immunoblots) were detectable in primary cultures of prostatic stromal and epithelial cells as well as in the immortalized nonmalignant prostatic human epithelial cells, P69, and in the P69 metastatic subline, M12. IGFBP-rP1 expression was not detectable in the prostatic cancer cell lines PC-3, DU145, and LNCaP. IGFBP-rP1 expression was regulated in P69 cells but not in M12 cells. Protein and mRNA expression was up-regulated by IGF-I, transforming growth factor-β, and retinoic acid. The observations that IGFBP-rP1 expression is significantly diminished in prostate tumorigenesis and is regulated in nonmalignant prostate cells suggest IGFBP-rP1 is important in normal prostatic cell growth.
AB - Insulin-like growth factor-binding protein-related protein-1 (IGFBP- rP1; also known as Mac25, TAF, and PSF) is a member of the IGFBP superfamily. It is a cystein-rich protein that shares structural and functional similarities with the conventional IGFBPs. In situ hybridization of prostate tissue sections Show intense IGFBP-rP1 messenger ribonucleic acid (mRNA) expression in normal stroma and glandular epithelium. There was a significant loss of detectable IGFBP-rP1 mRNA in metastatic prostate tissue. IGFBP-rP1 mRNA (Northern blots) and protein (immunoblots) were detectable in primary cultures of prostatic stromal and epithelial cells as well as in the immortalized nonmalignant prostatic human epithelial cells, P69, and in the P69 metastatic subline, M12. IGFBP-rP1 expression was not detectable in the prostatic cancer cell lines PC-3, DU145, and LNCaP. IGFBP-rP1 expression was regulated in P69 cells but not in M12 cells. Protein and mRNA expression was up-regulated by IGF-I, transforming growth factor-β, and retinoic acid. The observations that IGFBP-rP1 expression is significantly diminished in prostate tumorigenesis and is regulated in nonmalignant prostate cells suggest IGFBP-rP1 is important in normal prostatic cell growth.
UR - http://www.scopus.com/inward/record.url?scp=0032452436&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032452436&partnerID=8YFLogxK
U2 - 10.1210/jcem.83.12.5341
DO - 10.1210/jcem.83.12.5341
M3 - Article
C2 - 9851777
AN - SCOPUS:0032452436
SN - 0021-972X
VL - 83
SP - 4355
EP - 4362
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 12
ER -