Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours

Tan A. Ince; Aurea D. Sousa; Michelle A. Jones; J. Chuck Harrell; Elin S. Agoston; Marit Krohn; Laura M. Selfors; Wenbin Liu; Ken Chen; Mao Yong; Peter Buchwald; Bin Wang; Katherine S. Hale; Evan Cohick; Petra Sergent; Abigail Witt; Zhanna Kozhekbaeva; Sizhen Gao; Agoston T. Agoston; Melissa A. Merritt; Rosemary Foster; Bo R. Rueda; Christopher P. Crum; Joan S. Brugge; Gordon B. Mills

doi:10.1038/ncomms8419

Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours

Tan A. Ince, Aurea D. Sousa, Michelle A. Jones, J. Chuck Harrell, Elin S. Agoston, Marit Krohn, Laura M. Selfors, Wenbin Liu, Ken Chen, Mao Yong, Peter Buchwald, Bin Wang, Katherine S. Hale, Evan Cohick, Petra Sergent, Abigail Witt, Zhanna Kozhekbaeva, Sizhen Gao, Agoston T. Agoston, Melissa A. MerrittRosemary Foster, Bo R. Rueda, Christopher P. Crum, Joan S. Brugge, Gordon B. Mills

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

Currently available human tumour cell line panels consist of a small number of lines in each lineage that generally fail to retain the phenotype of the original patient tumour. Here we develop a cell culture medium that enables us to routinely establish cell lines from diverse subtypes of human ovarian cancers with >95% efficiency. Importantly, the 25 new ovarian tumour cell lines described here retain the genomic landscape, histopathology and molecular features of the original tumours. Furthermore, the molecular profile and drug response of these cell lines correlate with distinct groups of primary tumours with different outcomes. Thus, tumour cell lines derived using this methodology represent a significantly improved platform to study human tumour pathophysiology and response to therapy.

Original language	English (US)
Article number	7419
Journal	Nature communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms8419
State	Published - Jun 17 2015
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Ince, T. A., Sousa, A. D., Jones, M. A., Harrell, J. C., Agoston, E. S., Krohn, M., Selfors, L. M., Liu, W., Chen, K., Yong, M., Buchwald, P., Wang, B., Hale, K. S., Cohick, E., Sergent, P., Witt, A., Kozhekbaeva, Z., Gao, S., Agoston, A. T., ... Mills, G. B. (2015). Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours. Nature communications, 6, Article 7419. https://doi.org/10.1038/ncomms8419

Ince, TA, Sousa, AD, Jones, MA, Harrell, JC, Agoston, ES, Krohn, M, Selfors, LM, Liu, W, Chen, K, Yong, M, Buchwald, P, Wang, B, Hale, KS, Cohick, E, Sergent, P, Witt, A, Kozhekbaeva, Z, Gao, S, Agoston, AT, Merritt, MA, Foster, R, Rueda, BR, Crum, CP, Brugge, JS & Mills, GB 2015, 'Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours', Nature communications, vol. 6, 7419. https://doi.org/10.1038/ncomms8419

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title = "Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours",

abstract = "Currently available human tumour cell line panels consist of a small number of lines in each lineage that generally fail to retain the phenotype of the original patient tumour. Here we develop a cell culture medium that enables us to routinely establish cell lines from diverse subtypes of human ovarian cancers with >95% efficiency. Importantly, the 25 new ovarian tumour cell lines described here retain the genomic landscape, histopathology and molecular features of the original tumours. Furthermore, the molecular profile and drug response of these cell lines correlate with distinct groups of primary tumours with different outcomes. Thus, tumour cell lines derived using this methodology represent a significantly improved platform to study human tumour pathophysiology and response to therapy.",

author = "Ince, {Tan A.} and Sousa, {Aurea D.} and Jones, {Michelle A.} and Harrell, {J. Chuck} and Agoston, {Elin S.} and Marit Krohn and Selfors, {Laura M.} and Wenbin Liu and Ken Chen and Mao Yong and Peter Buchwald and Bin Wang and Hale, {Katherine S.} and Evan Cohick and Petra Sergent and Abigail Witt and Zhanna Kozhekbaeva and Sizhen Gao and Agoston, {Agoston T.} and Merritt, {Melissa A.} and Rosemary Foster and Rueda, {Bo R.} and Crum, {Christopher P.} and Brugge, {Joan S.} and Mills, {Gordon B.}",

note = "Funding Information: We would like to acknowledge Ferenc Reinhardt and Terri Woo for their technical assistance with mouse xenograft experiments and immunohistochemistry, respectively. We would like to thank Benjamin G. Neel, Christopher J. Kemp, Carla Grandori and Ulker Ince for critical comments, reading and edits of the manuscript. This work was funded by grants to T.A.I. from Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami; Breast Cancer Research Foundation, New York, NY; DoD-CDMRP Ovarian Cancer Research Program (W81XWH-14-1-0160) Resource Development Grant (OC130649); and National Cancer Institute, Roadmap Epigenomics project (R01-CA146445-01). Additional funding from Advanced Medical Research Foundation (B.R.R.), the Dr Miriam and Sheldon D. Adelson Medical Research Foundation (J.S.B.), CCSG grant P30 CA016672 (G.B.M.) and R01CA123219 to G.B.M., J.S.B. and T.A.I. Publisher Copyright: {\textcopyright} 2015 Macmillan Publishers Limited. All rights reserved.",

year = "2015",

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day = "17",

doi = "10.1038/ncomms8419",

language = "English (US)",

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journal = "Nature communications",

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T1 - Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours

AU - Ince, Tan A.

AU - Sousa, Aurea D.

AU - Jones, Michelle A.

AU - Harrell, J. Chuck

AU - Agoston, Elin S.

AU - Krohn, Marit

AU - Selfors, Laura M.

AU - Liu, Wenbin

AU - Chen, Ken

AU - Yong, Mao

AU - Buchwald, Peter

AU - Wang, Bin

AU - Hale, Katherine S.

AU - Cohick, Evan

AU - Sergent, Petra

AU - Witt, Abigail

AU - Kozhekbaeva, Zhanna

AU - Gao, Sizhen

AU - Agoston, Agoston T.

AU - Merritt, Melissa A.

AU - Foster, Rosemary

AU - Rueda, Bo R.

AU - Crum, Christopher P.

AU - Brugge, Joan S.

AU - Mills, Gordon B.

N1 - Funding Information: We would like to acknowledge Ferenc Reinhardt and Terri Woo for their technical assistance with mouse xenograft experiments and immunohistochemistry, respectively. We would like to thank Benjamin G. Neel, Christopher J. Kemp, Carla Grandori and Ulker Ince for critical comments, reading and edits of the manuscript. This work was funded by grants to T.A.I. from Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami; Breast Cancer Research Foundation, New York, NY; DoD-CDMRP Ovarian Cancer Research Program (W81XWH-14-1-0160) Resource Development Grant (OC130649); and National Cancer Institute, Roadmap Epigenomics project (R01-CA146445-01). Additional funding from Advanced Medical Research Foundation (B.R.R.), the Dr Miriam and Sheldon D. Adelson Medical Research Foundation (J.S.B.), CCSG grant P30 CA016672 (G.B.M.) and R01CA123219 to G.B.M., J.S.B. and T.A.I. Publisher Copyright: © 2015 Macmillan Publishers Limited. All rights reserved.

PY - 2015/6/17

Y1 - 2015/6/17

N2 - Currently available human tumour cell line panels consist of a small number of lines in each lineage that generally fail to retain the phenotype of the original patient tumour. Here we develop a cell culture medium that enables us to routinely establish cell lines from diverse subtypes of human ovarian cancers with >95% efficiency. Importantly, the 25 new ovarian tumour cell lines described here retain the genomic landscape, histopathology and molecular features of the original tumours. Furthermore, the molecular profile and drug response of these cell lines correlate with distinct groups of primary tumours with different outcomes. Thus, tumour cell lines derived using this methodology represent a significantly improved platform to study human tumour pathophysiology and response to therapy.

AB - Currently available human tumour cell line panels consist of a small number of lines in each lineage that generally fail to retain the phenotype of the original patient tumour. Here we develop a cell culture medium that enables us to routinely establish cell lines from diverse subtypes of human ovarian cancers with >95% efficiency. Importantly, the 25 new ovarian tumour cell lines described here retain the genomic landscape, histopathology and molecular features of the original tumours. Furthermore, the molecular profile and drug response of these cell lines correlate with distinct groups of primary tumours with different outcomes. Thus, tumour cell lines derived using this methodology represent a significantly improved platform to study human tumour pathophysiology and response to therapy.

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Characterization of twenty-five ovarian tumour cell lines that phenocopy primary tumours

Abstract

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