TY - JOUR
T1 - Chromosomal position or virus mutation permits retrovirus expression in embryonal carcinoma cells
AU - Barklis, Eric
AU - Mulligan, Richard C.
AU - Jaenisch, Rudolf
N1 - Funding Information:
This work was supported by grants HD-19105 from the National Institutes of Health and POICA38497 from the National Cancer Institute. E. B. was supported as the Syntex Fellow of the Life Sciences Research Foundation.
PY - 1986/11/7
Y1 - 1986/11/7
N2 - Retrovirus expression is restricted in embryonal carcinoma (EC) cells. To study how a virus can overcome this block, we selected and analyzed rare proviruses that are expressed in F9 cells. Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5′-flanking DNA sequences. Surprisingly, five proviruses in 17 selected cell lines integrated into the same two distinct chromosomal regions, suggesting that the number of chromosomal positions in the cellular genome that allows virus expression is very limited. Our results suggest that genomic sequences that are actively transcribed in EC cells can be isolated by selection for retrovirus expression.
AB - Retrovirus expression is restricted in embryonal carcinoma (EC) cells. To study how a virus can overcome this block, we selected and analyzed rare proviruses that are expressed in F9 cells. Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5′-flanking DNA sequences. Surprisingly, five proviruses in 17 selected cell lines integrated into the same two distinct chromosomal regions, suggesting that the number of chromosomal positions in the cellular genome that allows virus expression is very limited. Our results suggest that genomic sequences that are actively transcribed in EC cells can be isolated by selection for retrovirus expression.
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U2 - 10.1016/0092-8674(86)90596-9
DO - 10.1016/0092-8674(86)90596-9
M3 - Article
C2 - 3768959
AN - SCOPUS:0023007610
SN - 0092-8674
VL - 47
SP - 391
EP - 399
JO - Cell
JF - Cell
IS - 3
ER -