TY - JOUR
T1 - cis,cis-Cyclohexane 1,3,5-triol polyphosphates release calcium from Neurospora crassa via an unspecific ins 1,4,5-P3 receptor
AU - Schultz, C.
AU - Gebauer, G.
AU - Metschies, T.
AU - Rensing, L.
AU - Jastorff, B.
N1 - Funding Information:
ACKNOWLEDGMENTS: The authors would like to thank Dr. P. van Haastert, University of Groningen, for support in testing the bovine receptor, and Dr. M. Vicker for critically reading the manuscript. This work was supported by a grant of the Deutsche Forschungsge-meinschaft (Ja 246/5-2).
PY - 1990/2/14
Y1 - 1990/2/14
N2 - We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2+-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P2) did not induce Ca2+-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P3 analogogues.
AB - We investigated the effects of new inositol 1,4,5-trisphosphate analogues on the release of Ca2+ from isolated vacuoles of Neurospora crassa. Tri-O-butyryl-inositol 1,4,5-trisphosphate and a set of cis,cis-cyclohexane 1,3,5-triol bis-(CHT-P2) and trisphosphates (CHT-P3) gave an increase in free Ca2+ as measured directly with fura-2, a Ca2+-chelator. However, inositol 1,4-bisphosphate, 6-O-palmitoyl-inositol 4,5-bisphosphate and trans-cyclohexane 1,2-diol bisphosphate (trans CHD-P2) did not induce Ca2+-release. These results suggest that the 1,5-bisphosphate position in inositol 1,4,5-trisphosphate (Ins 1,4,5-P3) is the only essential arrangement for receptor binding to vacuoles of Neurospora crassa. The structures of these analogues are discussed on the basis of a general concept for the design of new Ins 1,4,5-P3 analogogues.
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U2 - 10.1016/0006-291X(90)91010-P
DO - 10.1016/0006-291X(90)91010-P
M3 - Article
C2 - 2154977
AN - SCOPUS:0025190174
SN - 0006-291X
VL - 166
SP - 1319
EP - 1327
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -