TY - JOUR
T1 - Cloning and expression of a heme binding protein from the genome of Saccharomyces cerevisiae
AU - Auclair, Karine
AU - Huang, Hong Wei
AU - Moënne-Loccoz, Pierre
AU - Ortiz De Montellano, Paul R.
N1 - Funding Information:
We thank Joseph DeRisi for providing the plasmid containing the gene encoding a mutated YLR205c, and Giselle Knudsen and Luke Lightning for their assistance with database searches and predictions. The work at the University of California in San Francisco was supported by the National Institutes of Health Grant DK30297.
PY - 2003/4/1
Y1 - 2003/4/1
N2 - The YLR205c gene of Saccharomyces cerevisiae does not show significant sequence identity to any known gene, except for heme oxygenase (22% to human HO-1). The YLR205 ORF was cloned and overexpressed in both Escherichia coli and S. cerevisiae. Both expression systems yielded proteins that bound heme tightly. The isolated YLR205c protein underwent reduction in the presence of either NADPH-cytochrome P450 reductase or NADH-putidaredoxin-putidaredoxin reductase but did not exhibit heme oxygenase activity. The protein exhibited modest H2O2-dependent peroxidase activities with guaiacol, potassium iodide, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Thus, YLR205c codes for a hemoprotein of unkown physiological function that exhibits peroxidase activity.
AB - The YLR205c gene of Saccharomyces cerevisiae does not show significant sequence identity to any known gene, except for heme oxygenase (22% to human HO-1). The YLR205 ORF was cloned and overexpressed in both Escherichia coli and S. cerevisiae. Both expression systems yielded proteins that bound heme tightly. The isolated YLR205c protein underwent reduction in the presence of either NADPH-cytochrome P450 reductase or NADH-putidaredoxin-putidaredoxin reductase but did not exhibit heme oxygenase activity. The protein exhibited modest H2O2-dependent peroxidase activities with guaiacol, potassium iodide, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS). Thus, YLR205c codes for a hemoprotein of unkown physiological function that exhibits peroxidase activity.
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U2 - 10.1016/S1046-5928(02)00699-X
DO - 10.1016/S1046-5928(02)00699-X
M3 - Article
C2 - 12699699
AN - SCOPUS:0038395861
SN - 1046-5928
VL - 28
SP - 340
EP - 349
JO - Protein Expression and Purification
JF - Protein Expression and Purification
IS - 2
ER -